| 引用本文: | 冯丰凑,李清,邱颖姮,邬晓鸥,李曙光.蔗糖硬脂酸酯中游离蔗糖的HPLC-ELSD法测定[J].中国现代应用药学,2014,31(1):93-95. |
| feng feng cou,li qing,qiu ying heng,wu xiao ou,li shu guang.Determination of Free Sucrose in Sucrose Stearate by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(1):93-95. |
|
| 摘要: |
| 目的 建立了高效液相色谱法测定蔗糖硬脂酸酯中的游离蔗糖。方法 以氨基键合硅胶为填充剂,流动相A为醋酸铵溶于乙腈中配制成10μg?mL?1的溶液；流动相B为醋酸铵溶于四氢呋喃-水(90:10)的混合溶液中配制成10μg?mL?1的溶液,梯度洗脱,蒸发光散射检测器。结果 蔗糖在0.25 ~2.5 mg?mL?1浓度范围内线性关系良好。回收率为100.5%,RSD为0.8%。结论 该方法灵敏度高、专属性强,可用于蔗糖硬脂酸酯中游离蔗糖的测定。 |
| 关键词: 蔗糖硬脂酸酯 游离蔗糖 高效液相色谱 蒸发光散射检测 测定 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Free Sucrose in Sucrose Stearate by HPLC-ELSD |
|
feng feng cou,li qing,qiu ying heng,wu xiao ou,li shu guang
|
|
shenzhen institute for drug control
|
| Abstract: |
| OBJECTIVE An HPLC-ELSD method was established for the determination of the free sucrose in sucrose stearate. METHODS A NH2 column was used with mobile phase A of 10 μg?mL?1 ammonium acetate in acetonitrile and mobile phase B of 10 μg?mL?1 ammonium acetate in tetrahydrofuran - water (90:10) by gradient elution. Evaporative light scattering detector was used. RESULTS The calibration curve of sucrose was linear in the concentration range of 0.25 – 2.5 mg?mL?1. The average recovery was 100.5%, with RSD of 0.8%. CONCLUSION The method was sensitive, selective, and could be used for the determination of free sucrose in sucrose stearate. |
| Key words: sucrose stearate free sucrose HPLC evaporative light scattering detector determination |
