| 引用本文: | 刘阳,郭伟英.葡聚糖凝胶微柱结合高效液相色谱法测定奥沙利铂脂质体包封率[J].中国现代应用药学,2014,31(1):74-77. |
| LIU Yang,GuoWeiying.Determination of Entrapment Efficiency of Oxaliplatin Liposomes by Sephadex Microcolumn combined with HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(1):74-77. |
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| 摘要: |
| 目的：对奥沙利铂脂质体进行质量评价,建立奥沙利铂脂质体包封率的测定方法。方法：采用凝胶微柱离心法分离游离药物与脂质体,以HPLC法测定奥沙利铂的药物含量,计算脂质体的包封率。结果：奥沙利铂浓度在0.02~1mg.mL-1范围内线性关系良好(r=0.9999),结果3批次奥沙利铂脂质体的包封率分别为56.6%,57.5%,60.0%。结论：该方法简便、迅速,可准确地测定出奥沙利铂脂质体的包封率。 |
| 关键词: 奥沙利铂 脂质体 HPLC 包封率 微柱离心法 |
| DOI： |
| 分类号:R944.9 |
| 基金项目: |
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| Determination of Entrapment Efficiency of Oxaliplatin Liposomes by Sephadex Microcolumn combined with HPLC |
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LIU Yang1, GuoWeiying2
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1.College of Pharmacy,Liaoning Medical University;2.Liaoning Medical University
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| Abstract: |
| OBJECTIVE To evaluate the quality of oxaliplatin liposomes, and to establish a method to determine the entrapment efficiency of oxaliplatin liposomes. METHODS The microcolumn centrifugation method was employed to separate the free drug from the oxaliplatin liposomes. The content of oxaliplatin was determined by HPLC to calculate the entrapment efficiency. RESULTS A calibrated linear curve of oxaliplatin concentration was within 0.02~1.0mg.mL-1(r=0.9999). The results showed that the average entrapment efficiencies of three batches of oxaliplatin liposomes were 56.6%, 57.5% and 60.0%, respectively. CONCLUSION The method was simple and rapid, and it can be used to determine the entrapment efficiency of oxaliplatin liposomes accurately. |
| Key words: oxaliplatin liposome HPLC entrapment efficiency microcolumn gel centrifugation |
