| 引用本文: | 俞发荣,连秀珍,张红梅,李世平,谢明仁.十二碳烯酸对人H4细胞毒性作用研究[J].中国现代应用药学,2014,31(2):134-136. |
| YU Farong,LIAN Xiuzhen,ZHANG Hongmei,LI Shiping,XIE Mingren.Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(2):134-136. |
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| 摘要: |
| 目的 探讨十二碳烯酸对人H4细胞的毒性作用机制。方法 采用MTT法、流式细胞仪检测十二碳烯酸对人H4细胞的毒性作用。结果 分别给予人H4细胞0.3，0.9，2.7 mg·L-1十二碳烯酸培养48 h，十二碳烯酸对人H4细胞增殖抑制率分别为41.64%，70.97%，81.52%，半数抑制浓度(IC50)为0.40 mg·L-1，与对照组相比，差异有统计学意义(P<0.01)；凋亡率分别为10.16%，18.22%，24.06%。结论 十二碳烯酸对人H4细胞具有显著的毒性作用，其机制可能与损伤线粒体结构，抑制细胞分裂等有关。 |
| 关键词: 十二碳烯酸 人H4细胞 毒性作用 |
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| 基金项目:兰州市科技计划资助项目(2010-1-128) |
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| Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells |
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YU Farong, LIAN Xiuzhen, ZHANG Hongmei, LI Shiping, XIE Mingren
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Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China
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| Abstract: |
| OBJECTIVE To study 2-dodecenoic acid toxicity mechanism on human H4 cells. METHODS Toxic effects of 2-dodecenoic acid on human H4 cells were detected with methods of MTT and flow cytometry. RESULTS Given the human H4 cells 0.3, 0.9, 2.7 mg·L-1 2-dodecenoic acid for 48 h, human H4 cells proliferation inhibition rates were 41.64%, 70.97%, 81.52%, respectively, and the 50% inhibitory concentration(IC50) was 0.40 mg·L-1, compared with the control group, the difference was statistically significant(P<0.01); apoptosis rates were 10.16%, 18.22%, 24.06%, respectively. CONCLUSION 2-Dodecenoic acid on human H4 cells has significant toxic effects, its mechanism may be related to the destruction of the cell mitochondrial structure and inhibition of cell division. |
| Key words: 2-dodecenoic acid human H4 cells toxic effects |
