| 引用本文: | 安军永,秦拢,杨丽萍,李晓燕,马艳玲.津力达颗粒薄层鉴别与定量测定研究[J].中国现代应用药学,2014,31(2):213-217. |
| AN Junyong,QIN Long,YANG Liping,LI Xiaoyan,MA Yanling.Study on TLC and Quantitative Determination of Jinlida Granule[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(2):213-217. |
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| 摘要: |
| 目的 制定津力达颗粒的快速质量控制方法。方法 采用TLC在4块薄层板上鉴别人参、丹参、葛根、黄连、知母、制何首乌及淫羊藿。用高效液相色谱法，以氨基键合硅胶为填充剂、乙腈-无水乙醇-2%磷酸溶液(84∶7∶9)为流动相，测定样品中苦参碱的含量。结果 通过方法学考察，苦参碱的进样量在87.6~3 650 ng(r=0.999 9)，与峰面积呈良好的线性关系。苦参碱平均回收率为100.4%(n=9)，RSD为0.98%。TLC鉴别阴性无干扰。结论 本方法简便、快捷、实用，可用于津力达颗粒的质量评价。 |
| 关键词: 津力达颗粒 薄层色谱法 苦参碱 高效液相色谱法 |
| DOI： |
| 分类号:R917 |
| 基金项目: |
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| Study on TLC and Quantitative Determination of Jinlida Granule |
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AN Junyong1, QIN Long1, YANG Liping2, LI Xiaoyan1, MA Yanling1
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1.Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China;2.Shijiazhuang Medical College, Shijiazhuang 050081, China
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| Abstract: |
| OBJECTIVE To establish a rapid method for the quality control of Jinlida granule. METHODS Ginseng Radix et Rhizoma, Salviae Miltiorrhizae Radix et Rhizoma, Puerariae Lobatae Radix, Coptidis Rhizoma, Anemarrhenae Rhizoma, Polygoni Multiflori Radix Praeparata and Epimedii Folium were identified by TLC on four thin-layer chromatographic plates. The content of matrine in samples was determined by using amino chemically bonded silica gel as the stationary phase, a mixture of acetonitrile, ethanol absolute and 2% phosphoric(84∶7∶9) as the mobile by HPLC. RESULTS The methodological study showed that a good linear correlation existed in the ranges of 87.6-3 650 ng(r=0.999 9) for matrine. The average recovery of matrine was 100.4%, RSD was 0.98%. The negative sample did not have interference on TLC identification. CONCLUSION The method of quality control is simple, rapid, feasible and applicable to comprehensive evaluation the quality of Jinlida granules. |
| Key words: Jinlida granule TLC matrine HPLC |
