| 摘要: |
| 目的 建立中成药、保健食品中非法添加的10种抗风湿类化学成分的快速测定方法。方法 采用高效液相色谱法进行初步筛查。色谱柱：Diamonsil C18(4.6 mm×250 mm，5 μm)，流动相：乙腈-0.02 mol·L-1醋酸铵溶液，梯度洗脱；流速：1.0 mL·min-1，检测波长：240 nm。结果 10种化学药得到了完全分离，检出限<2 ng。结论 本方法操作简单、快速，灵敏度高，能满足快速筛查抗风湿类中成药及保健品中非法添加化学药的需要。 |
| 关键词: 中成药 保健品 高效液相色谱法 快速筛查 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Screening for 10 Anti-rheumatism Chemical Components in Health Food and Chinese Traditional Medicine by HPLC |
|
LIAO Yu
|
|
Dongguan Institute For Food and Drug Control, Dongguan 523109, China
|
| Abstract: |
| OBJECTIVE To establish a method for rapid detection of 10 anti-rheumatism chemical components adding illegally in health food and traditional Chinese medicine. METHODS Using HPLC to conduct the preliminary screening. The Diamonsil C18(4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.02 mol·L-1 ammonium acetate with linear gradient elution at a flow rate of 1.0 mL·min-1, the detection wavelength was 240 nm. RESULTS These ten components were separated completely, and the LODs were <2 ng.CONCLUSION The method is simple, sensitive and quickly to detect chemical components in health food and Chinese Traditional Medicine. |
| Key words: traditional Chinese medicines health food HPLC rapid detection |
