| 引用本文: | 童晔玲,杨锋,戴关海,任泽明,王波波.猫爪草总皂苷体外抗人非小细胞肺癌NCI-H460细胞活性研究[J].中国现代应用药学,2013,30(11):1182-1186. |
| TONG Yeling,YANG Feng ,DAI Guanhai,REN Zeming,WANG Bobo.Study on the in Vitro Activity of Ranunculus Ternati Radix Saponins on Non-Small Cell Lung Cancer Cell of NCI-H460[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(11):1182-1186. |
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| 摘要: |
| 目的 观察猫爪草总皂苷体外对人非小细胞肺癌NCI-H460细胞活性的影响。方法 采用系统溶剂法提取猫爪草总皂苷，3H-TdR掺入法观察猫爪草总皂苷对NCI-H460细胞增殖的影响；流式细胞仪检测其对NCI-H460细胞凋亡和周期的影响。结果 猫爪草总皂苷对NCI-H460细胞增殖有较好的抑制作用，呈现较好的量效关系，可促进NCI-H460细胞的早期凋亡，细胞周期出现G0/G1期的阻滞。结论 猫爪草总皂苷能较好的抑制NCI-H460细胞增殖，其机制可能与诱导凋亡、G0/G1期阻滞有关。 |
| 关键词: 猫爪草总皂苷 人非小细胞肺癌NCI-H460细胞 细胞凋亡 细胞周期 |
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| 基金项目:浙江省中医药管理局资助项目(2011ZB010) |
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| Study on the in Vitro Activity of Ranunculus Ternati Radix Saponins on Non-Small Cell Lung Cancer Cell of NCI-H460 |
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TONG Yeling, YANG Feng , DAI Guanhai, REN Zeming, WANG Bobo
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Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To observe the in vitro activity of Ranunculus Ternati Radix saponins(RRTS) on non-small cell lung cancer(NSCLC) cell of NCI-H460. METHODS Ranunculus Ternati Radix saponins was extracted by the technique of solvent-refining. Effect of different concentrations of Ranunculus Ternati Radix saponins on cell NCI-H460 were tested by 3H-TdR incorporation method. The cell cycle phase and apoptosis were inspected by flow cytometry. RESULTS The results showed that RRTS could inhibit the growth of NCI-H460 cell, showing a dose-effect curve. It could also promote the early apoptosis of cell NCI-H460 and arrest the cell cycle in G0/G1 phase. CONCLUSION Ranunculus Ternati Radix saponins can inhibit the proliferation of cell NCI-H460, its mechanism is probably related to apoptosis induction and blockage of cell cycle in G0/G1 phase. |
| Key words: Ranunculus Ternati Radix saponins NCI-H460 apoptosis cell cycle |
