| 引用本文: | 刘影,杨菁,王晶,刘涛.HPLC-ELSD测定地黄寡糖中地黄苷A含量[J].中国现代应用药学,2013,30(11):1223-1225. |
| LIU Ying,YANG Jing ,WANG Jing,LIU Tao.Determination of Rehmannioside A in Rehmannia Glutinosa Oligosaccharide by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(11):1223-1225. |
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| 摘要: |
| 目的 建立测定地黄寡糖中地黄苷A的高效液相色谱法。方法 采用Agilent TC C18色谱柱(4.6 mm×250 mm，5 μm)，柱温35 ℃，流动相：甲醇-水(10∶90)，流速1.0 mL·min-1；ELSD参数：漂移管温度110 ℃,载气流量3.2 L·min-1。结果 地黄苷A在0.2~1.2 mg·mL-1(r=0.999 3)内线性关系良好，平均回收率为98.8%(RSD=1.6%)。结论 该方法准确、简便、专属性好，可用于地黄寡糖中地黄苷A成分的含量测定，为地黄寡糖的质量控制提供了依据。 |
| 关键词: 地黄寡糖 地黄苷A 高效液相色谱法 蒸发光散射检测器 |
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| 基金项目:辽宁省自然科学基金(20072202)；辽宁省教育厅资助课题(20060528) |
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| Determination of Rehmannioside A in Rehmannia Glutinosa Oligosaccharide by HPLC-ELSD |
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LIU Ying1, YANG Jing 1, WANG Jing2, LIU Tao1
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1.Liaoning Medical University, Faculty of Pharmacy, Jinzhou 121000, China;2.Liaoning Medical University, First Affiliated Hospital, Jinzhou 121000, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide. METHODS The HPLC-ELSD was used with Agilent TC C18 column (4.6 mm×250 mm, 5 μm) at 35 ℃. The mobile phase was methanol and water(10∶90) with a flow rate 1.0 mL·min-1; The temperature of the drift tube in ELSD was 110 ℃ and the flow rate of dry air was 3.2 L·min-1. RESULTS The rehmannioside A had a good linear relationship between 0.2-1.2 mg·mL-1. CONCLUSION The method is accurate, simple with good specificity，and it could be used to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide, which may be providing a evidence for the quality control of Rehmannia glutinosa oligosaccharide. |
| Key words: Rehmannia glutinosa oligosaccharide rehmannioside A HPLC ELSD |
