| 引用本文: | 时向民,李天德,王玉堂,单兆亮,杨庭树.外源性磷酸肌酸对缺血豚鼠心室肌细胞钠通道的影响Δ[J].中国现代应用药学,2014,31(1):13-17. |
| shi xiangmin,li tiande,wang yutang,shan zhaoliang,yang tingshu.Effect of exogenous phosphocreatine on natrium current in ischemic ventricular myocytes of guinea pig[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(1):13-17. |
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| 摘要: |
| 目的：观察不同浓度外源性磷酸肌酸(exogenous phosphocreatine, PCr)对豚鼠缺血心室肌细胞钠通道(INa)电流的影响,探讨其预防缺血性心律失常的电生理学机制。方法：心室肌细胞经酶解从豚鼠左心室获得,膜片钳全细胞模式记录INa电流,通过灌注模拟缺血液并充以95%N2 5%CO2的混合气体建立缺血模型,将PCr加入模拟缺血液中分别配成5, 10, 20,30 mmol/L浓度。将细胞分成6组,分别予模拟缺血液,含有5, 10, 20,30mmol/L浓度PCr的模拟缺血液,台氏液灌流,后者充以95%O2 5%CO2的混合气体。10min后记录各组的峰电流及电流密度。结果：与台氏液组相比,单纯模拟缺血液组INa峰电流密度降低80.1?2.5%(p<0.05),含有5, 10, 20, 30mmol/L浓度PCr的模拟缺血液组INa峰电流密度分别降低56.2?4.6%(p<0.05)；30.3?5.3%(p<0.05)；39.0?5.5%(p<0.05)；42.6?4.8%(p<0.05)。10与5, 20, 30mmol/L之间具有统计学差异(p<0.05)。结论： PCr能增加缺血时受抑制的INa峰电流及电流密度,这可能是其预防缺血性心律失常的电生理学机制。PCr在低浓度 (0~10mmol/L)对INa峰电流及电流密度的影响呈现明显的量效关系。 |
| 关键词: 膜片钳 钠电流 缺血 磷酸肌酸 豚鼠 |
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| 基金项目:解放军总医院苗圃基金(项目编号：08MP13) |
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| Effect of exogenous phosphocreatine on natrium current in ischemic ventricular myocytes of guinea pig |
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shi xiangmin1, li tiande2, wang yutang2, shan zhaoliang2, yang tingshu2
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1.hainan branch of PLA hospital;2.the general hospital of PLA
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| Abstract: |
| Objective: The aim of this study is to determine the effect of exogenous phosphocreatine(PCr) with different concentration on natrium ( INa ) current in guinea pig ischemic ventricular myocytes and to explore the antiarrhythmia mechanism in the treatment of ischemic heart disease. Methods: Ventricular myocytes were isolated enzymatically from left ventricular of guinea pig. Peak INacurrent was recorded using patch clamp techniques in the whole-cell configuration when myocytes had been superfused with normal Tyrode solution, simple ischemic solution, ischemic solution containing PCr with different concentration of 5, 10, 20, 30 mmol/L for 10 minutes respectively. Results: Peak INa current density of myocytes superfused with simple simulated ischemic solution was significantly reduced by 80.1?2.5%(p<0.05)compared with myocytes superfused with normal Tyrode solution. Ischemic solution containing PCr of 5, 10, 20, 30mmol/L reduced peak INa current density by 56.2?4.6%(p<0.05)；30.3?5.3%(p<0.05)；39.0?5.5%(p<0.05)；42.6?4.8%(p<0.05)respectively. There was Statistical significance among ischemic solution containing PCr of 10 and 5, 20, 30 mmol/L(p<0.05)。Conclusion: PCr could reverse the inhibition of INacurrent under ischemic condition, which could be the mechanism responsible for arrhythmia prevention in ischemia heart disease. 0~10mmol/L PCr exerted significant dose-effect relationship and concentration more than 10mmol/L could slightly decrease INa current density. |
| Key words: Patch clamp Natrium current Ischemia Phosphocreatine Guinea pig |
