| 引用本文: | 赵祥升,董娜,冯锦东,杨美华.益智多糖含量测定[J].中国现代应用药学,2013,30(10):1070-1074. |
| ZHAO Xiangsheng,DONG Na,FENG Jindong,YANG Meihua.Determination of Polysaccharides Content in Alpinia Oxyphylla[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(10):1070-1074. |
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| 摘要: |
| 目的 研究益智中多糖含量的测定方法。方法 水提醇沉法提取粗多糖，Sevage法除蛋白，H2O2脱色，流水透析和冷冻干燥制备多糖。1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生，HPLC测定其单糖组成。以益智精制多糖测得多糖对葡萄糖的换算因子，苯酚-硫酸法测定益智中多糖的含量。结果 益智多糖由葡萄糖、鼠李糖、半乳糖、甘露糖和阿拉伯糖5种单糖组成。益智多糖含量为12.91%，RSD=4.72%(n=5)，平均加样回收率为99.1%，RSD为4.57%(n=5)。结论 该法简便，快捷，可用于益智多糖的含量测定。 |
| 关键词: 益智 多糖 单糖 含量测定 |
| DOI： |
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| 基金项目:中央公益性基金项目(2011HNB04)；海南省中药现代化专项项目(2010ZY011) |
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| Determination of Polysaccharides Content in Alpinia Oxyphylla |
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ZHAO Xiangsheng1, DONG Na2, FENG Jindong1, YANG Meihua1,3
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1.Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences, Wanning 571533, China;2.Life Science and Technology Department, Henan Institute of Science and Technology, Xinxiang 453003, China;3.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
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| Abstract: |
| OBJECTIVE To establish a method for content determination of polysaccharides in Alpinia oxyphylla. METHODS The crude polysaccharides were extracted by hot water and precipitated with ethanol. The purified polysaccharide was obtained by removing protein and colors with Sevage and H2O2, repectively, then run water dialysis and frozen-drying. The monosaccharide composition was determined by HPLC after precolum-derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP). The conversion coefficient of Alpinia oxyphylla polysaccharides to glucose was obtained by refined polysaccharides, and then the content of crude polysaccharides in Alpinia oxyphylla was determined by sulfuric acid-phenol method. RESULTS The monosaccharide compositions in polysaccharides from Alpinia oxyphylla were Glu, Rha, Gal, Man, and Ara. The content of polysaccharides was 12.91%(RSD=4.72%, n=5), and the average recovery was 99.1% with RSD of 4.57%(n=5). CONCLUSION The method is sample, and can be used for determination of the content of polysaccharides from Alpinia oxyphylla. |
| Key words: Alpinia oxyphylla polysaccharide monosaccharide content determination |
