水杨酸和牛血清白蛋白的相互作用研究及共存铜离子的影响

王旭; 吴淑春; 王家学; 郑青; 郭玉华

引用本文:	王旭,吴淑春,王家学,郑青,郭玉华.水杨酸和牛血清白蛋白的相互作用研究及共存铜离子的影响[J].中国现代应用药学,2013,30(10):1066-1070.
	WANG Xu,WU Shuchun,WANG Jiaxue,ZHENG Qing,GUO Yuhua.Interaction of Salicylic Acid with Bovine Serum Albumin and the Effect of Coexistent Cu²⁺ on the Reaction[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(10):1066-1070.

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水杨酸和牛血清白蛋白的相互作用研究及共存铜离子的影响
王旭¹, 吴淑春¹, 王家学¹, 郑青², 郭玉华²
1.浙江医学高等专科学校，杭州 310053;2.湖州师范学院生命科学学院，浙江湖州 313000

摘要:

目的研究铜离子对水杨酸与血清白蛋白相互作用的影响。方法采用荧光光谱技术研究了不同温度下水杨酸与牛血清白蛋白(BSA)的相互作用及铜离子对水杨酸-BSA体系的影响。结果运用理论模型处理实验数据，得到水杨酸与牛血清白蛋白(BSA)的猝灭常数、结合常数、结合力类型等相互作用参数。结论铜离子存在时，不改变水杨酸对BSA内源荧光的猝灭类型和水杨酸-BSA分子间作用的类型，但使水杨酸与BSA的表观结合常数增大。

关键词: 水杨酸牛血清白蛋白铜离子荧光光谱法

DOI：

分类号:

基金项目:浙江省自然科学基金项目(LQ12B03001)；浙江医学高等专科学校一般研究计划项目(2013B04)

Interaction of Salicylic Acid with Bovine Serum Albumin and the Effect of Coexistent Cu²⁺ on the Reaction

WANG Xu¹, WU Shuchun¹, WANG Jiaxue¹, ZHENG Qing², GUO Yuhua²

1.Zhejiang Medical College, Hangzhou 310053, China;2.Faculty of Life Science, Huzhou Teachers College, Huzhou 313000, China

Abstract:

OBJECTIVE To investigate the interactions of salicylic acid and bovine serum albumin (BSA) in the presence of Cu²⁺. METHODS The interaction between salicylic acid and BSA was investigated using fluorescence at different temperatures. The effect of Cu²⁺ on the salicylic acid-BSA system was also researched. RESULTS The quenching constants, binding constants and binding force type were measured according to theory model. CONCLUSION The presence of Cu²⁺ doesn’t change quenching type and force type between salicylic acid and BSA, but the apparent association constant(KLB) becomes higher.

Key words: salicylic acid bovine serum albumin Cu²⁺ fluorescence spectrometry