| 引用本文: | 杨道纳,蔡天进,刘金来,李笑慧,周曙华.HPLC测定中医滋补膏方中橙皮苷含量[J].中国现代应用药学,2013,30(5):511-513. |
| YANG Daona,CAI Tianjin,LIU Jinlai,LI Xiaohui,ZHOU Shuhua.Determination of Hesperidin in Herbal Paste by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(5):511-513. |
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| 摘要: |
| 目的 建立高效液相色谱法测定中医滋补膏方中橙皮苷含量的方法。方法 采用Hypersil ODS2色谱柱(250 mm×4.6 mm，5 mm)；乙腈-甲酸缓冲液(取甲酸1.7 mL，加水，再加三乙胺1.8 mL，定容至1 000 mL)(20∶80)为流动相；流速为1.0 mL·min-1；检测波长为283 nm，柱温为30 ℃，进样量为20 mL。结果 橙皮苷2.1~12.6 mg·mL-1内，峰面积与浓度呈良好的线性关系(r=0.999 8)，平均回收率和RSD分别为99.1%和1.68%。结论 方法简便、可靠，重复性好，可用于中医滋补膏方中橙皮苷含量测定。 |
| 关键词: 高效液相色谱法 含量测定 中医滋补膏方 橙皮苷 |
| DOI： |
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| 基金项目:2011年浙江省药学会医院药学科研专项基金项目(2011ZYY17) |
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| Determination of Hesperidin in Herbal Paste by HPLC |
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YANG Daona1, CAI Tianjin1, LIU Jinlai2, LI Xiaohui2, ZHOU Shuhua2
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1.Cangnan People's Hospital, Cangnan 325800, China;2.Wenzhou People's Hospital, Wenzhou 325000, China
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| Abstract: |
| OBJECTIVE To establish a high performance liquid chromatography method for the determination of the Hesperidin in Herbal Paste. METHODS The analytical column was packed with the Hypersil ODS2 column(250 mm×4.6 mm, 5 μm). Mobile phase consisted of Acetonitrile-Methanoic acid buffer solutions(20∶80), The flow rate was 1.0 mL·min-1 and the UV detection wavelength was 283 nm. The temperature of the column was 30 ℃. The injection volume was 20 μL. RESULTS A good linearity was obtained over the range of 2.1-12.6 μg·mL-1 for Hesperidin(r=0.999 8). The average recovery was 99.1% and RSD was 1.68%. CONCLUSION This method is simple, reliable, reproducible and can be used for the determination of Hesperidin in this preparation. |
| Key words: HPLC determination herbal paste hesperidin |
