| 引用本文: | 朱兰寸,刘志华,李高,杨光忠,马廷升.微乳液相色谱法测定广东紫珠中5种黄酮成分[J].中国现代应用药学,2013,30(6):651-654. |
| ZHU Lancun,LIU Zhihua,LI Gao,YANG Guangzhong,MA Tingsheng.Simultaneous Determination of Five Flavonoids in Callicarpa Kwangtungensis Chun. by Microemulsion Liquid Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(6):651-654. |
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| 摘要: |
| 目的 建立微乳液相色谱法同时测定广东紫珠中5,4′-二羟基-3,7,3′-三甲氧基黄酮、鼠李秦素、华良姜素、岳桦素、毡毛美洲茶素5种黄酮成分。方法 微乳体系组成的流动相为：2.2%SDS -1.5%正辛烷-7.75%正丁醇- 0.5%三乙胺-88%水(用H3PO4调节pH 3.7)，色谱柱为Hypersil BDS C18(4.6 mm×150 mm，5 μm)柱；检测波长350 nm；流速0.8 mL·min-1；进样量10 μL；柱温为30 ℃。结果 广东紫珠中5种黄酮成分在30 min内达到完全分离，在1.520~72.25 μg·mL-1内，5个黄酮成分的线性相关系数r≥0.999 5，平均回收率99.6％~100.5％。结论 本方法可应用于中广东紫珠中5种黄酮成分的含量分析。 |
| 关键词: 微乳液相色谱法 广东紫珠 黄酮 |
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| 基金项目:湖南省教育厅自然科学基金资助项目(11C1012) |
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| Simultaneous Determination of Five Flavonoids in Callicarpa Kwangtungensis Chun. by Microemulsion Liquid Chromatography |
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ZHU Lancun1, LIU Zhihua1, LI Gao2, YANG Guangzhong3, MA Tingsheng1
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1.Pharmacology Department of Huaihua Medical College, Huaihua 418000, China;2.Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China;3.Pharmacology Department of South-central University for Nationalities, Wuhan 430074, China
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| Abstract: |
| OBJECTIVE To establish an efficient microemulsion liquid chromatography(MELC) method and validate for simultaneous determination of five flavonoids (5,4′-dihydroxy-3,7,3′-trimethoxyflavone, rhamnatin, kumatakenin, ermanine and velutin) in Callicarpa kwangtungensis Chun.. METHODS The MELC separation was performed on a Hypersil BDS Cl8 column(4.6 mm×150 mm, 5 μm) with the mixture of 2.2% SDS-1.5% octane-7.75% n-butanol-0.5% triethylamine-88% water, the pH was adjusted to 3.7 with phosphoric acid as the mobile phase. The flow rate was set at 0.8 mL·min-1 and the eluent was detected at 350 nm for the five flavonoids．RESULTS The calibration curves of the five flavonoids were linear (r≥0.999 5) over the concentration range of 1.520-72.25 μg·mL-1. The mean recoveries were 99.6%-100.5%. CONCLUSION The results indicate that the optimized method is successfully applied to the analysis of five important flavonoids in Callicarpa kwangtungensis Chun.. |
| Key words: microemulsion liquid chromatography? Callicarpa kwangtungensis Chun.? flavonoid |
