| 引用本文: | 孙莲,孟磊,勉强辉,孟海强.HPLC同时测定新疆药桑枝中6种成分的含量[J].中国现代应用药学,2013,30(6):660-663. |
| SUN Lian,MENG Lie,MIAN Qianghui,MENG Haiqiang.Simultaneous Determination of Six Ingredients in Xingjiang Ramulus Mori by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(6):660-663. |
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| 摘要: |
| 目的 建立HPLC同时测定新疆药桑枝中绿原酸、芦丁、异槲皮苷、紫云英苷、白藜芦醇和桑色素含量的方法。方法 采用PMC pack ODS 色谱柱(4.6 mm×250 mm，5 μm)，以0.2%磷酸水溶液-0.2%磷酸乙腈溶液为流动相，梯度洗脱，检测波长：360 nm，流速：0.6 mL·min-1。结果 6种成分在45 min内完全分离，6种成分的平均回收率为97.7%~100.7%(RSD≤2.0%)。结论 本方法操作简单、结果准确，具有较好的重复性和稳定性，为药桑枝的质量控制提供参考。 |
| 关键词: 药桑枝 紫云英苷 白藜芦醇 桑色素 高效液相色谱法 |
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| Simultaneous Determination of Six Ingredients in Xingjiang Ramulus Mori by HPLC |
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SUN Lian, MENG Lie, MIAN Qianghui, MENG Haiqiang
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Department of Chemistry, Pharmacy College of Xinjiang Medical University, Urumqi 830011, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid, rutin, isoquercitrin, astragalin, resveratrol and morin in Ramulus Mori. METHODS The separation was carried out on a PMC-pack ODS(4.6 mm×250 mm, 5 μm) column with 0.2% phosphoric acid-0.2% phosphoric acid in acetonitrile as a mobile phase at a flow rate of 0.6 mL·min-1 with a gradient program. The detection wavelength was 360 nm. RESULTS Six ingredients were completely separated within 45 min. The average recoveries were between 97.7% and 100.7%(RSD≤2.0%). CONCLUSION The method is simple, rapid, reproducibility, accurate and reliable which can be used as the reference for the quality control of Ramulus Mori. |
| Key words: Ramulus Mori astragalin resveratrol morin HPLC |
