| 引用本文: | 张海珠,李齐贤,孙帮燕,周萍.HPLC同时测定西归中阿魏酸和佛手柑内酯的含量[J].中国现代应用药学,2013,30(6):648-651. |
| ZHANG Haizhu,LI Qixian,SUN Bangyan,ZHOU Ping.Simultaneous Determination of Bergapten and Ferulic Acid in Seselopsis by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(6):648-651. |
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| 摘要: |
| 目的 建立同时测定西归药材中阿魏酸和佛手柑内酯含量的方法。方法 采用色谱柱为PhenomeneX Gemini-NX C18 110A柱(4.6 mm×250 mm，5 μm)；流动相为甲醇-0.05%磷酸水溶液，梯度洗脱；流速为1.0 mL·min-1；检测波长为322 nm；柱温为30 ℃。结果 阿魏酸在0.020 1~0.603 μg、佛手柑内酯在0.020 3~0.609 μg内线性关系良好；阿魏酸和佛手柑内酯的平均回收率分别为96.8%(RSD=2.0%)和98.7%(RSD=2.9%)。结论 本方法首次建立了同时测定西归中阿魏酸和佛手柑内酯的HPLC分析方法，结果准确，重复性好，可作为西归药材的质量评价方法。 |
| 关键词: 西归 阿魏酸 佛手柑内酯 含量测定 高效液相色谱法 |
| DOI： |
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| 基金项目:大理学院应用开发研究基金(YYKF2006-1-1) |
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| Simultaneous Determination of Bergapten and Ferulic Acid in Seselopsis by HPLC |
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ZHANG Haizhu, LI Qixian, SUN Bangyan, ZHOU Ping
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College of Pharmacy and Chemistry, Dali University, Dali 671000, China
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| Abstract: |
| OBJECTIVE To simultaneous determine the content of ferulic acid and bergapten in Seselopsis. METHODS The chromatographic separation was performed on a PhenomeneX Gemini-NX C18 110A (250 mm×4.60 mm, 5μm). The mobile phase was consisted of methanol-0.05% phosphoric acid with gradient elute, the flow rate was 1.0 mL·min-1. The detection wavelength was 322 nm and the column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of ferulic acid and bergapten was achieved when the amount was within 0.020 1-0.603 μg, 0.020 3-0.609 μg; the recoveries were 96.8% and 98.7%; RSD were 2.0% and 2.9%. CONCLUSION This is the first time to simultaneous determine ferulic acid and bergapten in Seselopsis. The method is accurate, reproducible and suitable to be used for quality assessment of Seselopsis. |
| Key words: Seselopsis ferulic acid bergapten content determination HPLC |
