| 引用本文: | 胡曼曼,胡国新 ,孙丽蓉,周曙华,熊建华.舒尼替尼与Combretastatin A-4联合用药体外抑制人胃癌SGC-7901细胞增殖作用研究[J].中国现代应用药学,2013,30(7):726-730. |
| HU Manman,HU Guoxin ,SUN Lirong,ZHOU Shuhua,XIONG Jianhua.Synergistic Anti-tumor activity of Sunitinib Combined with Combretastatin A-4 in SGC-7901 Cells by Apoptosis[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(7):726-730. |
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| 摘要: |
| 目的 对舒尼替尼与Combretastatin A-4(CA-4)联合用药的抗肿瘤活性进行研究。方法 SRB法测定比较舒尼替尼、CA-4及联合用药对人胃癌SGC-7901细胞增殖抑制作用。PI染色法、DAPI染色法及Western blot法检测舒尼替尼联合应用CA-4诱导人胃癌SGC-7901细胞凋亡作用。结果 联用舒尼替尼和CA-4在体外能协同抑制人胃癌SGC-7901细胞的增殖。舒尼替尼联用CA-4能够诱导SGC-7901肿瘤细胞凋亡，并且伴随着caspase-3的激活和PARP的裂解。结论 舒尼替尼与CA-4联合用药在体外能协同诱导人胃癌SGC-7901肿瘤细胞增殖。 |
| 关键词: 舒尼替尼 CA -4 协同作用 细胞凋亡 抗肿瘤活性 |
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| Synergistic Anti-tumor activity of Sunitinib Combined with Combretastatin A-4 in SGC-7901 Cells by Apoptosis |
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HU Manman1, HU Guoxin 2, SUN Lirong1, ZHOU Shuhua1, XIONG Jianhua1
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1.Departmnet of Pharmacy，Wenzhou Third Clinical College and Wenzhou People’s Hospital affiliated With Wenzhou Medical College, Wenzhou 325000, China;2.Departmnet of Clinical Pharmacy, Wenzhou Medical College, Wenzhou 325027, China
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| Abstract: |
| OBJECTIVE To study the anti-cancer activity of the combination of CA-4 and sunitinib. METHODS The anti-proliferation activity of CA-4 and sunitinib were analyzed by SRB, apoptosis was detected by PI, DAPI and Western blot analysis. RESULTS The present study indicated that the combination of sunitinib and CA-4 exerted synergistic anti-proliferative effects against human gastric cancer cells SGC-7901 in vitro. The enhanced apoptosis induced by sunitinib plus CA-4 was accompanied by the greater extent of caspases-3 activation and PARP cleavage in SGC-7901 cells. CONCLUSION These findings build the rationale for further clinical development of sunitinib and CA-4 against gastric cancer (SGC-7901). |
| Key words: sunitinib CA-4 synergy apoptosis anti-tumor activity |
