| 引用本文: | 罗金文,颜琳琦,李樱红,吴鸳鸯,倪维芳,陶巧凤.HPLC测定化妆品中非法添加的米诺地尔[J].中国现代应用药学,2013,30(6):655-657. |
| LUO Jinwen,YAN Linqi,LI Yinghong,WU Yuanyang,NI Weifang,TAO Qiaofeng.Determination of Minoxidil Illegally Adulterated in Cosmetics by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(6):655-657. |
|
| 摘要: |
| 目的 建立快速鉴定化妆品中非法添加的米诺地尔的检测方法。方法 以迪马C18柱(4.6 mm×250 mm，5 μm)为分析柱；采用甲醇-水-冰醋酸(730∶270∶10)(1 000 mL-1中含磺基丁二酸钠二辛酯3.0 g，并用高氯酸调节pH值至3.0)为流动相；流速为1.0 mL·min-1；检测波长为280 nm。结果 米诺地尔在1~100 μg·mL-1浓度内线性良好；最低检测限为5 μg·g-1；平均回收率100.4%。结论 本法快速准确、专属性强、灵敏度高，可用于育发类化妆品中米诺地尔的检测。 |
| 关键词: 米诺地尔 高效液相色谱法 化妆品 非法添加 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Minoxidil Illegally Adulterated in Cosmetics by HPLC |
|
LUO Jinwen, YAN Linqi, LI Yinghong, WU Yuanyang, NI Weifang, TAO Qiaofeng
|
|
Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China
|
| Abstract: |
| OBJECTIVE To establish a specific method for the determination of minoxidil illegally adulterated in cosmetics. METHODS The separation and analysis was performed on a Diamonsil C18 column(4.6 mm×250 mm, 5.0 μm). The mobile phase consisted of methanol-water-acetic acid glacial (730∶270∶10)(contained 0.3% of dioctyl sodium sulfosuccinate and adjusted pH to 3.0 with perchloric acid). The flow rate was 1.0 mL·min-1 and detected at 280 nm. RESULTS The assay linearity of minoxidil was confirmed over the range of 1-100 μg·mL-1, and the detection limit was 5 μg·g-1. The average recovery was 100.4%. CONCLUSION The method is proved to be accurate, specific, sensitive and convenient. Therefore, it can be used to determinate minoxidil illegally adulterated in hair cosmetics. |
| Key words: minoxidil HPLC cosmetics illegally adulterated |
