| 引用本文: | 施宪宝,单丽娜,郭斌,周盾,王桂军,冯玉军.HPLC手性固定相法测定患者血浆中布洛芬对映体的浓度[J].中国现代应用药学,2013,30(3):316-318. |
| SHI Xianbao,SHAN Lina ,GUO Bin,ZHOU Dun,WANG Guijun,FENG Yujun.Determination of Ibuprofen Enantiomers in Plasma by HPLC-Chiral Stationary Phase[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(3):316-318. |
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| 摘要: |
| 目的 建立用高效液相色谱法测定血浆中布洛芬对映体浓度的方法。方法 用正己烷-异丙醇混合液(100∶5)提取血浆样品中布洛芬，在Chirobiotic V(250 mm×4.6 mm，5 μm)柱上，以甲醇-冰醋酸-三乙胺(100∶0.04∶0.02)为流动相进行分离，于226 nm 波长处检测，内标为吲哚美辛。结果 R-布洛芬、S-布洛芬和内标没有干扰，分离度良好。R-布洛芬和S-布洛芬血药浓度在0.01~5 mg·L-1内线性关系良好。两个对映体相对回收率分别为99.1%~102.2%和99.3%~102.4%，绝对回收率分别为66.8~72.9和66.3~71.4，日内RSD分别为4.2%~5.4%和3.6%~5.1%，日间RSD分别为5.9%~7.3%和6.2%~7.9%。结论 该方法准确、快速、简便、灵敏度高、重复性好，可同时测量血浆中R-布洛芬和S-布洛芬的药物浓度，并可用于药动学参数的研究及患者血药浓度监测。 |
| 关键词: 高效液相色谱法 布洛芬 手性固定相 对映体 |
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| Determination of Ibuprofen Enantiomers in Plasma by HPLC-Chiral Stationary Phase |
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SHI Xianbao1, SHAN Lina 1, GUO Bin1, ZHOU Dun1, WANG Guijun1, FENG Yujun2
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1.The First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, China;2.Maternal and Child Care Center of Qinhuangdao, Qinhuangdao 066000, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of plasma levels of enantiomers of ibuprofen. METHODS Ibuprofen was extracted from samples with Hexane-isopropanol(100∶5). HPLC was performed on Chirobiotic column with methanol-aceticacid-triethylamine(100∶0.04∶0.02) as mobile phase. Detection wavelength was 226 nm. Indomethacin was used as the internal standard. RESULTS Under this chromatographic condition, R-ibuprofen, S-ibuprofen and indomethacin had no interference within each other. The calibration curve was linear in the range of 0.01-5 mg·L-1 for R-ibuprofen and S-ibuprofen. The relative recoveries of R-ibuprofen and S-ibuprofen were 99.1%-102.2% and 99.3%-102.4% respectively. The absolute recoveries of R-ibuprofen and S-ibuprofen were 66.8-72.9 and 66.3-71.4 respectively; RSD range of intraday were 4.2%-5.4% and 3.6%-5.1%. RSD ranges of interday were 5.9%-7.3% and 6.2%-7.9% respectively. CONCLUSION This method is accurate, rapid, with high sensitivity and good repeatability. It is applicable for the determination and pharmacokinetic study of the enantiomers of ibuprofen in plasma. |
| Key words: HPLC ibuprofen chiral stationary phase enantiomers |
