| 引用本文: | 王石健,俞振伟.HPLC测定热熔压敏胶贴剂中α-细辛醚的含量[J].中国现代应用药学,2013,30(2):191-193. |
| WANG Shijian,YU Zhenwei.Determination of α-Asarone in Hot Melt Pressure Sensitive Adhesive Patches by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(2):191-193. |
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| 摘要: |
| 目的 建立HPLC测定热熔压敏胶贴剂中α-细辛醚含量的方法。方法 以甲苯为萃取溶剂提取药物，采用ZORBAX Eclipse XDB-C18色谱柱(150 mm×4.6 mm，5 μm)，以甲醇-水(55∶45)为流动相，流速：1.0 mL·min-1，检测波长：311 nm，柱温：40 ℃。结果 α-细辛醚在8.032~18.07 μg·mL-1内线性关系良好(r=0.999 9)，回收率为99.3%(RSD=0.99%)，供试品溶液在室温下48 h内稳定。结论 本方法操作简单，结果准确，可用于热熔压敏胶贴剂中α-细辛醚的含量测定。 |
| 关键词: α-细辛醚 热熔压敏胶 贴剂 高效液相色谱法 |
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| Determination of α-Asarone in Hot Melt Pressure Sensitive Adhesive Patches by HPLC |
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WANG Shijian1, YU Zhenwei2
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1.Taizhou Municipal Hospital, Taizhou 318000, China;2.Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of α-asarone in hot melt pressure sensitive adhesive patches. METHODS Toluene was used as the exacted solvent. The column was ZORBAX Eclipse XDB-C18(150 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (55∶45) and the flow rate was 1 mL·min-1. The detection wave length was 311 nm. The column temperature was kept at 40 ℃. RESULTS A good linearity was obtained in a range of 8.032-18.07 μg·mL-1 (r=0.999 9), and the recovery was 99.3%(RSD=0.99%). The solution was stable within 48 h under room temperature. CONCLUSION The method is simple and accurate, and is suitable for the determination of α-asarone in hot melt pressure sensitive adhesive patches. |
| Key words: α-asarone hot melt pressure sensitive adhesive patch HPLC |
