| 引用本文: | 叶菲,楼雪芳,杜永忠.HPLC测定龙血竭脂质纳米粒中龙血素A的含量[J].中国现代应用药学,2013,30(1):69-72. |
| YE Fei,LOU Xuefang,DU Yongzhong.Determination of Loureirin A in Solid Lipid Nanoparticles Encapsulating Resina Draconis by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(1):69-72. |
|
| 摘要: |
| 目的 采用HPLC测定龙血竭脂质纳米粒中龙血素A的含量并计算其包封率。方法 采用DiamonsilTM C18(200 mm×4.6 mm，5 μm)色谱柱，以乙腈-1%冰醋酸水溶液(38∶62)为流动相，流速为1.3 mL·min-1，检测波长为260 nm，柱温为40 ℃，测定样品中龙血素A的含量并计算其包封率。结果 龙血素A 于0.135~5.4 μg·mL-1内有良好线性关系，Y=33.694X+1.589 8(r=0.999 9)。低、中、高3个浓度的日内RSD分别为2.70%，2.36%，1.48%；日间RSD分别为2.70%，2.61%，1.97%，该方法的平均回收率为99.2%，RSD为3.9%，平均包封率为89.59%。结论 本方法准确、简便、重复性好，可用于龙血竭脂质纳米粒中龙血素A含量的测定。 |
| 关键词: 高效液相色谱法 龙血竭 龙血素A 脂质纳米粒 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Loureirin A in Solid Lipid Nanoparticles Encapsulating Resina Draconis by HPLC |
|
YE Fei1, LOU Xuefang2, DU Yongzhong3
|
|
1.Hospital of Zhejiang University, Hangzhou 310058, China;2.Zhejiang University City College, Hangzhou 310015, China;3.College of Pharmaceutial Sciences, Zhejiang University, Hangzhou 310058, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of loureirin A content in solid lipid nanoparticles(SLN) of Resina Draconis. METHODS A DiamonsilTM C18(200 mm×4.6 mm, 5 μm) column was used for the determination of loureirin A with mobile phase of acetonitrile-1% acetic acid solution (38∶62). The flow rate was 1.3 mL·min-1, the detection wavelength was set at 260 nm, and the column temperature was 40 ℃. RESULTS The linear range of 1oureirin A was 0.135-5.4 μg·mL-1, Y=33.694X+1.589 8(r=0.999 9). The RSDs of within-day were 2.70%, 2.36%, 1.48%, and the RSDs of between-day were 2.70%, 2.61%, 1.97% in the high-, middle-, and low-dose Resina Draconis loaded SLN. The average recovery was 99.2%(RSD 3.9%), and the average drug encapsulation efficiency was 89.59%. CONCLUSION The method is simple and reliable for the determination of loureirin A in Resina Draconis loaded SLN. |
| Key words: HPLC Resina Draconis loureirin A solid lipid nanoparticle |
