| 引用本文: | 龙丽辉,郑艳侠,陈琳,徐利,曹永孝,刘静,韩燕.六肽对兔血小板聚集活性的影响[J].中国现代应用药学,2013,30(2):118-121. |
| LONG Lihui,ZHENG Yanxia,CHEN Lin,XU Li,CAO Yongxiao,LIU Jing,HAN Yan.Influence of Hexapeptide on Rabbit Platelet Aggregation Activity[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(2):118-121. |
|
| 摘要: |
| 目的 研究六肽对兔血小板聚集活性的影响。方法 采集健康家兔颈动脉血，以枸橼酸钠抗凝，用比浊法测其血小板在不同诱导剂诱导下聚集率。结果 1×10-5 mol·L-1六肽，对兔ADP、花生四烯酸(AA)和凝血酶诱导的血小板聚集的抑制率分别为(66.22±1.40)%，(67.94±2.32)%和(58.18±4.67)%。六肽抑制兔ADP、AA和凝血酶诱导的血小板聚集的IC50分别为3.24×10-6 mol·L-1，1.32×10-6 mol·L-1和7.24×10-6 mol·L-1。结论 六肽在体外具有抑制兔血小板聚集的作用。 |
| 关键词: 六肽 血小板聚集 二磷酸腺苷 花生四烯酸 凝血酶 |
| DOI： |
| 分类号: |
| 基金项目:陕西省教育厅项目(11JK0722)；西安医学院附属医院项目(XYFY11-02) |
|
| Influence of Hexapeptide on Rabbit Platelet Aggregation Activity |
|
LONG Lihui1,2, ZHENG Yanxia1, CHEN Lin1, XU Li1, CAO Yongxiao2, LIU Jing2, HAN Yan2
|
|
1.Department of Pharmacy, Affiliated Hospital of Xi’an Medical College, Xi’an 710077, China;2.Department of Pharmacology, Xi’an Jiaotong University College of Medicine, Xi’an 710061, China
|
| Abstract: |
| OBJECTIVE To investigate the effect of hexapeptide on platelet aggregation activity in rabbit. METHODS The blood of rabbit carotid artery was taken, and then, the anticoagulation blood was obtained by adding sodium citrate into the whole blood. The platelet aggregation rates induced by different inducers were determined by turbidimetry. RESULTS The aggregation inhibition rates of 1×10-5 mol·L-1 hexapeptide induced by ADP, arachidonic acid and thrombin in rabbit, were (66.22±1.40)%, (67.94±2.32)% and (58.18±4.67)%, respectively. The IC50 of aggregation inhibition rates of hexapeptide in rabbit induced by ADP, arachidonic acid and thrombin, were 3.24×10-6 mol·L-1, 1.32×10-6 mol·L-1 and 7.24×10-6 mol·L-1, respectively. CONCLUSION Hexapeptide has the effect of anti-platelet aggregation in rabbit in vitro. |
| Key words: hexapeptide platelet aggregation adenosine diphosphate arachidonic acid thrombin |
