| 引用本文: | 刘亚楠,王伟.HPLC测定扎鲁司特片中扎鲁司特的含量[J].中国现代应用药学,2013,30(2):178-179. |
| LIU Yanan,WANG Wei.Content Determination of Zafirlukast in Zafirlukast Tablets by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(2):178-179. |
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| 摘要: |
| 目的 建立高效液相色谱法测定扎鲁司特片中扎鲁司特的含量。方法 采用Zorbax Eclipse Plus C18柱(250 mm×4.6 mm，5 μm)，流动相为0.05 mol·L-1磷酸二氢钾溶液 (用磷酸调节pH至3.0)-乙腈(25∶75)，流速为1.0 mL·min-1，检测波长为242 nm。结果 扎鲁司特在20~70 μg·mL-1内与峰面积呈良好线性关系(r=0.999 7，n=6)，平均回收率为100.1%，RSD为0.61%。结论 该方法简便，准确，重复性好，可用于扎鲁司特片中扎鲁司特的质量控制。 |
| 关键词: 扎鲁司特 高效液相色谱法 含量测定 |
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| Content Determination of Zafirlukast in Zafirlukast Tablets by HPLC |
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LIU Yanan, WANG Wei
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Guangdong Zhongke Drug Research & Develop Limited Company, Guangzhou 510600, China
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| Abstract: |
| OBJECTIVE To establish a method for the content determination of zafirlukast in Zafirlukast Tablets by HPLC. METHODS Zorbax Eclipse Plus C18(250 mm×4.6 mm，5 μm) column was used with 0.05 mol·L-1 potassium dihydrogen phosphate (adjust pH to 3.0 with phosphoric acid)-acetonitrile (25∶75) as mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was 242 nm. RESULTS The linear rang of zafirlukast was 20-70 μg·mL-1(r=0.999 7, n=6), the average recovery was 100.1%, RSD was 0.61%. CONCLUSION The method is simple, reliable, accurate and can be used in the quality control of zafirlukast in Zafirlukast Tablets. |
| Key words: zafirlukast HPLC content determination |
