| 引用本文: | 王晶,李鑫.美洲大蠊提取物对人肝癌细胞Bel-7402作用机制的研究[J].中国现代应用药学,2012,29(10):876-880. |
| WANG Jing,LI Xin.Effects of Periplaneta Americana L. Extract on Human Hepatoma Cells Bel-7402[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(10):876-880. |
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| 摘要: |
| 目的 探讨美洲大蠊提取物对人肝癌细胞Bel-7402的作用机制。方法 MTT比色法观察美洲大蠊提取物对人肝癌细胞Bel-7402增殖的影响，AnnexinV-FITC/PI双染色法研究美洲大蠊提取物对人肝癌细胞Bel-7402凋亡的影响，流式细胞术检测线粒体膜电位，DNA Ladder实验检测细胞凋亡，蛋白印迹法检测Bax、Bcl-2、Caspase-9、Caspase-3、Caspase-8蛋白的表达。结果 美洲大蠊提取物可抑制人肝癌细胞Bel-7402增殖，IC50为28.2 μg·mL-1，并可诱导人肝癌细胞Bel-7402凋亡，降低线粒体膜电位。DNA Ladder实验可见明显的梯形电泳图谱，蛋白印迹法显示Bax、Caspase-9、Caspase-3蛋白表达增强，Bcl-2蛋白表达减弱，Caspase-8蛋白表达无明显变化。结论 美洲大蠊提取物可通过线粒体途径诱导人肝癌细胞Bel-7402凋亡。 |
| 关键词: 美洲大蠊提取物 人肝癌细胞Bel-7402 凋亡 线粒体途径 |
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| Effects of Periplaneta Americana L. Extract on Human Hepatoma Cells Bel-7402 |
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WANG Jing, LI Xin
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First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, China
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| Abstract: |
| OBJECTIVE To investigate the effects of Periplaneta americana L. extract on human hepatoma cells Bel-7402. METHODS The proliferation of human hepatoma cells Bel-7402 was observed by MTT colorimetric, apoptosis was studied by Annexin V-FITC/PI staining. The changes of mitochondrial membrane potential were monitored by flow cytometry, cell apoptosis was detected by DNA ladder experiments, and the protein expressions of Bax, Bcl-2, Caspase-9, Caspase-3, Caspase-8 were tested by western blotting test. RESULTS Periplaneta americana L. extract inhibited the proliferation of human hepatoma cells Bel-7402, IC50 was 28.2 μg·mL-1. It also induced the apoptosis of human hepatoma cells Bel-7402 and reduced the mitochondrial membrane potential. DNA ladder experiments showed trapezoidal electrophoretogram. Western blotting test showed that the protein expressions of Bax, caspase-9, caspase-3 increased, Bcl-2 decreased, and Caspase-8 expression was not significantly changed. CONCLUSION Periplaneta americana L. extract induces the apoptosis of human hepatoma cells Bel-7402 through mitochondrial pathway. |
| Key words: Periplaneta americana L. extract human hepatoma cells Bel-7402 apoptosis mitochondrial pathway |
