| 引用本文: | 吴明钗，沈秀微，林洁，谢丽晓，黄鹏，易林高，林思思.HPLC测定氟康唑莫西沙星眼用即型凝胶的含量[J].中国现代应用药学,2012,29(6):534-536. |
| WU Mingchai, SHEN Xiuwei, LIN Jie, XIE Lixiao, HUANG Peng, YI Lingao, LIN Sisi.Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(6):534-536. |
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| 摘要: |
| 目的 建立高效液相色谱法测定氟康唑莫西沙星眼用即型凝胶含量，同时测定制剂中氟康唑、莫西沙星和羟苯乙酯的含量。方法 采用ODS-C18色谱柱，以三乙胺缓冲液-甲醇为流动相，流速1.0 mL·min-1，检测波长260 nm。结果 氟康唑、莫西沙星、羟苯乙酯的线性范围分别为25~250 mg·mL-1(r=0.999 9，n=6)，25~250 mg·mL-1(r=0.999 9，n=6)，2.5~25 mg·mL-1(r=0.999 7，n=6)，平均回收率分别为99.6%，99.4%，99.3%。RSD分别为1.23%，1.20%，1.17%。结论 本方法操作简便，灵敏度高，重复性好，可用于该制剂的含量测定。 |
| 关键词: 高效液相色谱法 含量测定 氟康唑 莫西沙星 羟苯乙酯 |
| DOI： |
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| 基金项目:温州市科技计划项目(Y20110193) |
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| Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC |
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WU Mingchai, SHEN Xiuwei, LIN Jie, XIE Lixiao, HUANG Peng, YI Lingao, LIN Sisi1,2
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1.The Third Affiliated Hospital of Wenzhou Medical College, Rui’an 325200, China;2.Wenzhou Medical College, Wenzhou 325000, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C18 column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min-1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25-250 μg·mL-1(r=0.999 9, n=6), 25-250 μg·mL-1(r=0.999 9, n=6), 2.5-25 μg·mL-1 (r=0.999 7, n=6), the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation. |
| Key words: HPLC content determination fluconazole moxifloxacin ethylparaben |
