| 引用本文: | 江松敏,蔡琳,朱婷婷,施立华,金芩,夏清海.FGF21对HepG2细胞TGF-β信号通路的影响[J].中国现代应用药学,2012,29(8):675-678. |
| JIANG Songmin,CAI Lin,ZHU Tingting,SHI Lihua,JIN Qin,XIA Qinghai.Effect of FGF21 in TGF-β Signaling Pathway of HepG2 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(8):675-678. |
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| 摘要: |
| 目的 研究FGF21对TGF-b/Smads信号通路中相关蛋白和mRNA表达的影响。方法 采用Western blot法检测HepG2 cells的TGF-b1，TGF-b RⅡ，Smad 2，3，4，7的蛋白表达水平和采用Q-PCR方法检测HepG2 cells的TGF-b1，TGF-b RⅡ，Smad 2，3，4，7的mRNA表达水平。结果 在不同FGF21浓度下，TGF-b1，TGF-b RⅡ，Smad 2，3，4，7蛋白和mRNA表达水平不同(P<0.05)。结论 FGF21通过促进TGF-β信号通路中的信号分子或受体的表达，或者通过下调此信号通路的阻断分子而抑制肿瘤发生。 |
| 关键词: FGF21 HepG2细胞 Smad |
| DOI： |
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| 基金项目:浙江省教育厅科研项目(Y200907097) |
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| Effect of FGF21 in TGF-β Signaling Pathway of HepG2 Cells |
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JIANG Songmin, CAI Lin, ZHU Tingting, SHI Lihua, JIN Qin, XIA Qinghai
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School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China
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| Abstract: |
| OBJECTIVE To study the effects of FGF21 on relative proteins and mRNA expression involved in TGF-b/Smads signaling pathway. METHODS Detect the protein expressions of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 of HepG2 cells with Western blot and detect the mRNA expression of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 of HepG2 cells with Q-PCR. RESULTS The expressions of the protein and mRNA of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 varied with the different concentrations of FGF21(P<0.05). CONCLUSION FGF21 might inhibit tumor by promoting TGF-β signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking molecules of this signaling pathway. |
| Key words: FGF21 HepG2 cells Smad |
