| 引用本文: | 罗敏敏,李春华,王易芬.HPLC测定野马追中野马追内酯A，B和金丝桃苷的含量[J].中国现代应用药学,2012,29(11):1018-1022. |
| LUO Minmin,LI Chunhua,WANG Yifen.Determination of Eupalinolide A, B and Hyperin in Eupatorium Lindleyanum DC by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(11):1018-1022. |
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| 摘要: |
| 目的 采用高效液相色谱法对不同产地的6批野马追药材中野马追内酯A，B和金丝桃苷进行了定性定量分析。方法 野马追内酯A，B的测定采用Agilent Extend-C18(4.6 mm×150 mm，5 μm)为分析柱，乙腈-水(27∶73)为流动相，流速为1.0 mL·min-1，等度洗脱，检测波长为220 nm，柱温为30 ℃。金丝桃苷的测定以SB-C18为分析柱，乙腈-1%醋酸水溶液(12∶88)为流动相，流速为1.0 mL·min-1，等度洗脱，检测波长为255 nm。结果 野马追内酯A在0.02~1.88 μg，野马追内酯B在0.44~4.44 μg内与峰面积呈良好的线性关系。金丝桃苷在0.101~1.414 μg内与峰面积呈良好的线性关系。结论 本实验方法准确、快速、灵敏、重复性好，在该色谱条件及样品处理方法下，可以准确测定野马追中野马追内酯A，B和金丝桃苷的含量。 |
| 关键词: 野马追 野马追内酯A 野马追内酯B 金丝桃苷 高效液相色谱法 |
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| 基金项目:中国科学院昆明植物所植物化学与西部植物资源持续利用国家重点实验室课题(P2010-ZZ06) |
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| Determination of Eupalinolide A, B and Hyperin in Eupatorium Lindleyanum DC by HPLC |
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LUO Minmin1, LI Chunhua1, WANG Yifen2
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1.School of Pharmaceutical Engineering, Central South University, Changsha 410083, China;2.Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of the content of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC from 6 different areas by HPLC. METHODS For eupalinolide A, B, Agilent Extend-C18 column (4.6 mm×150 mm, 5 μm) was used under the mobile phase of acetonitrile-water(27∶73) at the flow rate of 1.0 mL·min-1. The detection wavelength was 220 nm and the column temperature was maintained at 30 ℃. For hyperin, SB-C18 column was used under the mobile phase of acetonitrile-1% Acetic acid(12∶88) at a flow of 1.0 mL·min-1. The detection wavelength was 255 nm and the column temperature was maintained at 30 ℃. RESULTS The standard curve of eupalinolide A was linear in the range of 0.02-1.88 μg and eupalinolide B was linear in the range of 0.44-4.44 μg. The standard curve of hyperin was linear in the range of 0.101-1.414 μg. CONCLUSION This method was simple, accurate and reproducible for the determination of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC. |
| Key words: Eupatorium lindleanum DC. eupalinolide A eupalinolide B hyperin HPLC |
