| 引用本文: | 崔永霞,吴明侠,许闽,王丽果.不同产地野菊花中绿原酸、4, 5-二咖啡酰奎尼酸的含量测定[J].中国现代应用药学,2012,29(9):799-801. |
| CUI Yongxia,WU Mingxia,XU Min,WANG Liguo.Content Determination of Chlorogenic Acid and 4,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos from Different Areas[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(9):799-801. |
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| 摘要: |
| 目的 建立不同产地野菊花中绿原酸、4,5-二咖啡酰奎尼酸的含量测定方法。方法 采用HPLC，色谱柱为Diamonsil C18(4.6 mm×250 mm，5 μm)；采用梯度洗脱；检测波长327 nm；柱温30 ℃；流速1.0 mL·min-1。结果 绿原酸、4,5-二咖啡酰奎尼酸的线性范围分别为0.011~0.22 μg，0.017 5~0.35 μg。加样回收率分别为99.1%和98.8%；RSD分别为1.93%和1.54%(n=9)。结论 本方法操作简便，分析速度快，结果准确，重复性好，可用于野菊花中绿原酸和4,5-二咖啡酰奎尼酸含量的测定。 |
| 关键词: 野菊花 高效液相色谱法 绿原酸 4,5-二咖啡酰奎尼酸 |
| DOI： |
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| 基金项目:河南中医学院博士科研基金(BSJJ2009-11) |
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| Content Determination of Chlorogenic Acid and 4,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos from Different Areas |
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CUI Yongxia, WU Mingxia, XU Min, WANG Liguo
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Henan College of TCM, Zhengzhou 450008, China
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| Abstract: |
| OBJECTIVE To determine the content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos from different areas. METHODS HPLC was used. The chromatographic separation was performed on a Diamonsil C18(4.6 mm × 250 mm, 5 μm) column with gradient elute. The detection wavelength was 327 nm and column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid was achieved when the amount was within 0.011-0.22 μg, 0.0175-0.35 μg; the recoveries were 99.1% and 98.8%; RSD were 1.93% and 1.54%(n=9). CONCLUSION The method is accurate, reproducible and suitable to determine chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos. |
| Key words: Chrysanthemi Indici Flos HPLC chlorogenic acid 4,5-O-dicaffeoylquinic acid |
