| 引用本文: | 刘元,宋志钊,文志云.HPLC测定翅茎白粉藤中白黎芦醇的含量[J].中国现代应用药学,2012,29(8):734-736. |
| LIU Yuan,SONG Zhizhao,WEN Zhiyun.Determination of Resveratrol in Cissus Hexangularis by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(8):734-736. |
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| 摘要: |
| 目的 建立翅茎白粉藤中白黎芦醇的含量测定方法。方法 采用高效液相色谱法，色谱柱XB-C18(4.6 mm×250 mm，5 μm)，以乙腈-水(25∶75)为流动相，检测波长为306 nm，柱温为室温。结果 白黎芦醇在2.50~40.0 μg内峰面积与其浓度呈良好线性关系(r=0.999 9)，平均回收率为97.3%，RSD为0.98%(n=6)。结论 本方法简便、准确、重复性好，可作为该药材含量测定方法。 |
| 关键词: 翅茎白粉藤 高效液相色谱法 白黎芦醇 |
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| 基金项目:广西科技攻关项目(桂科攻0992003A-22)；广西壮药质量标准(第二卷)标准研究项目(MZY2010054)；广西壮族自治区卫生厅自筹经费科研课题(Z2011276) |
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| Determination of Resveratrol in Cissus Hexangularis by HPLC |
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LIU Yuan, SONG Zhizhao, WEN Zhiyun
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Guangxi Institute of Chinese Medicine & Pharmaceutical Scince, Nanning 530022, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for detecting resveratrol in Cissus hexangularis. METHODS An XB-C18 column(4.6 mm×250 mm, 5 mm) was used. The mobile phase consisted of acetonitrile-water(25∶75). The detection wavelength was 306 nm and the column temperature was room temperature. RESULTS Resveratrol showed a good linear relationship in the range of 2.50-40.0 μg·mL-1, r=0.999 9. The recovery and RSD were 97.5% and 0.98%(n=6), respectively. CONCLUSION This method is simple, accurate and repeatable to determine the content of resveratrol in Cissus hexangularis. |
| Key words: Cissus hexangularis HPLC resveratrol |
