| 引用本文: | 李博,杨兴官,李红玉.HPLC同时测定复方替硝唑载体栓中3组分含量[J].中国现代应用药学,2012,29(11):1015-1018. |
| LI Bo,YANG Xingguan,LI Hongyu.Simultaneous Determination of Three Components in Compound Tinidazole Carrier Suppositories by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(11):1015-1018. |
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| 摘要: |
| 目的 建立HPLC同时测定复方替硝唑载体栓中3组分含量。方法 采用Diamonsil C18(200 mm×4.6 mm，5 μm)；流动相：甲醇-水-三乙胺(60∶40∶0.5)(用磷酸调pH值至4.0)，流速：1.0 mL·min-1，检测波长：260 nm，进样量：20 μL；柱温：25 ℃。结果 复方替硝唑载体栓中3组分替硝唑、克霉唑、醋酸氯己定的线性范围分别为40~600，32~480，1.6~24 μg·mL-1(r=0.999 9)，平均回收率分别为99.81％，100.2％，100.3％；RSD分别为0.37％，0.2％和0.4％(n=9)。结论 该方法可同时测定3种成分含量，操作简便、快速，结果准确、可靠。 |
| 关键词: 复方替硝唑载体栓 替硝唑 克霉唑 醋酸氯己定 高效液相色谱法 |
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| 基金项目:辽宁省级科学技术研究成果奖(20080599) |
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| Simultaneous Determination of Three Components in Compound Tinidazole Carrier Suppositories by HPLC |
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LI Bo, YANG Xingguan, LI Hongyu
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Department of Pharmacy, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method to simultaneous determine three components in compound tinidazole carrier suppositories. METHODS A Diamonsil-C18(200 mm×4.6 mm, 5 μm) column was used; the mobile phase was the mixture of methanol-water-triethylamine(60∶40∶0.5)(adjusted by phosphoric acid to pH 4.0), the flow rate was 1.0 mL·min-1, the UV detector was operated at 260 nm, injection volume was 20 μL; column temperature was 25 ℃. RESULTS The linear ranges of three components in compound tinidazole carrier suppositories tinidazole, clotrimazole, chlorhexidine acetate were 40–600, 32–480, 1.6–24 μg·mL-1(r=0.999 9). The average recoveries respectively were 99.81%, 100.2%, 100.3%, and RSDs respectively were 0.37%, 0.2% and 0.4%(n=9). CONCLUSION This method can simultaneously determine three components. It is easy and fast with accurate and reliable results. |
| Key words: compound tinidazole carrier suppositories tinidazole clotrimazole chlorhexidine acetate HPLC |
