| 引用本文: | 侯巍，朱小庆，楚婧，高金波.硒化玉米须多糖的工艺条件及硒含量测定研究[J].中国现代应用药学,2012,29(7):621-624. |
| HOU Wei, ZHU Xiaoqing, CHU Jing, GAO Jinbo.Study on the Synthesis Processing and Content Determination of Corn Silk Polysaccharide Selenide[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(7):621-624. |
|
| 摘要: |
| 目的 以玉米须多糖为原料，用亚硒酸钠进行玉米须多糖的硒化研究。方法 利用单因素和正交试验确立硒化的最佳工艺条件；利用硒-硫氰酸钾-甲基紫萃取光度法测定硒多糖中的硒含量，并通过红外光谱对硒多糖进行了初步表征。结果 最佳工艺条件为反应温度70 ℃，反应时间8 h，玉米须多糖与亚硒酸钠质量比为1∶1.2，硝酸体积分数为0.3%，玉米须硒多糖中硒含量为3.17 mg·g-1，平均收率为35.72%。红外光谱显示：玉米须硒多糖中含有Se=O键和Se-C键。结论 利用该工艺成功合成了玉米须硒多糖，为玉米须的开发和利用奠定基础。 |
| 关键词: 玉米须多糖 亚硒酸钠 硒化 正交试验 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Study on the Synthesis Processing and Content Determination of Corn Silk Polysaccharide Selenide |
|
HOU Wei, ZHU Xiaoqing, CHU Jing, GAO Jinbo
|
|
The Key Laboratory for Biological Drug of the Education Department Helongjiang, College of Pharmacy, Jiamusi University, Jiamusi 154007, China
|
| Abstract: |
| OBJECTIVE Using the polysaccharide from corn silk as the raw material, to study the selenizing process with sodium selenite. METHODS Selenizing conditions were established by single factor and orthogonal design; determining content of polysaccharide selenide by Se(Ⅳ)-KSCN-MV extraction spectrophotometry; characterizing the structure of polysaccharide selenide by IR. RESULTS The optimal conditions were: 70 ℃ reaction temperature, 8 hours reaction time, mass ratio of corn silk polysaccharide to sodium selenite 1∶1.2, 0.3% HNO3 of volume fraction. The average content of selenium in corn silk polysaccharide was over 3.17 mg·g-1 and average yield was 35.72%. IR results showed that corn silk polysaccharide contained Se=O and Se-C. CONCLUSION Corn silk polysaccharide selenide is successfully synthesized and the study provides the foundation for further study and exploiting for corn silk. |
| Key words: corn silk polysaccharide sodium selenite selenizing orthogonal test |
