| 引用本文: | 王大力，狄斌，李龙，程明川.银杏叶片中银杏黄酮在Beagle犬体内的药动学研究[J].中国现代应用药学,2012,29(6):483-486. |
| WANG Dali, DI Bin, LI Long, CHENG Mingchuan.Pharmacokinetics of Ginkgo Flavones in Ginkgo Leaf Tablets in Beagle Dogs[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(6):483-486. |
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| 摘要: |
| 目的 以槲皮素为代表，研究银杏叶片中银杏黄酮类成分在Beagle犬体内的药动学特征。方法 Beagle犬口服给药银杏叶片后，按设定时间静脉采血。血样经固相萃取、酸水解后，以HPLC-ECD测定不同时间点血浆中槲皮素的浓度。应用DAS数据处理软件对数据进行统计学处理。结果 口服给药后，槲皮素在Beagle犬体内的药动学过程符合二房室模型，其主要药动学参数为t1/2z 5.67 h，AUC0-t 63.8 mg·L-1·h，Cmax 15.9 mg·L-1。结论 建立的方法简单、灵敏、准确, 可用于研究以槲皮素为代表的银杏黄酮类成分的药动学特征，为银杏叶片的临床应用提供了依据。 |
| 关键词: 银杏黄酮 槲皮素 高效液相色谱-电化学检测法 药动学 |
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| Pharmacokinetics of Ginkgo Flavones in Ginkgo Leaf Tablets in Beagle Dogs |
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WANG Dali, DI Bin, LI Long, CHENG Mingchuan1,2
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1.The Central Hospital of Huzhou of Zhejiang, Huzhou 313000, China;2.China Pharmaceutical University, Nanjing 210009, China
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| Abstract: |
| OBJECTIVE To investigate pharmacokinetics of ginkgo flavones in Beagle dogs by quercetin, a typical aglycone of ginkgo flavones. METHODS Blood samples were collected at scheduled time points after a single oral administration of ginkgo leaf tablets. The concentration of quercetin in plasma was determined by HPLC-ECD after solid-phase extraction (SPE) and hydrolyzed by HCl. The main parameters of pharmacokinetics were calculated by DAS software. RESULTS The concentration-time course of quercetin was best fitted to a two-compartment model after oral administration. The main pharmacokinetic parameters were t1/2z 5.67 h, AUC0-t 63.8 mg·L-1·h, Cmax 15.9 mg·L-1. CONCLUSION The method is simple, sensitive and accurate. It provides an important method for the determination and pharmacokinetics study of quercetin. |
| Key words: ginkgo flavones quercetin HPLC-ECD pharmacokinetics |
