| 引用本文: | 农小英,黄礼德,郭立强,潘廷啟,颜祖弟,张照平,黄锁义.纤维素酶法提取土人参多糖的工艺研究[J].中国现代应用药学,2012,29(9):802-804. |
| NONG Xiaoying,HUANG Lide,GUO Liqiang,PAN Tingqi,YAN Zudi,ZHANG Zhaoping,HUANG Suoyi.Research on the Extraction Process of Polysaccharide from Talinum Crassifolium Using Cellulase[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(9):802-804. |
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| 摘要: |
| 目的 优化纤维素酶法提取土人参多糖。方法 通过单因素实验和正交实验，研究pH值、酶用量、酶解温度和提取时间对土人参多糖提取效果的影响。结果 纤维素酶法提取的优化工艺条件为：pH值为6，每克药材酶用量为400 U，酶解温度50 ℃，提取时间3 h。在此条件下，土人参多糖的提取率平均为21.16％，RSD为0.14％。结论 纤维素酶法提取土人参多糖效果省时高效。 |
| 关键词: 土人参 多糖 纤维素酶 正交设计 |
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| 基金项目:2013年度广西自治区级大学生创新训练计划立项资助项目(QJCX201338)；右江民族医学院民族医药协会资助项目(20110715) |
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| Research on the Extraction Process of Polysaccharide from Talinum Crassifolium Using Cellulase |
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NONG Xiaoying, HUANG Lide, GUO Liqiang, PAN Tingqi, YAN Zudi, ZHANG Zhaoping, HUANG Suoyi
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Youjiang Medical University for Nationalities, Baise 533000, China
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| Abstract: |
| OBJECTIVE To opmtimize the extraction technique for total polysaccharide from Talinum Crassifolium. METHODS The single factor experiment and the orthogonal design were conducted to investigate the influence of pH, enzyme dosage, extraction temperature and extraction time on yield of Polysaccharide from Talinum Crassifolium. RESULTS The optimum conditions of cellulose enzyme were as follows: pH 6.0, enzyme dosage 400 U·g-1 enzymes drug, temperature 50 ℃, extraction time 3 h, and extraction yield was 21.16, RSD was 0.14%. CONCLUSION Extraction polysaeeharide from Talinum Crassifolium by cellulose enzyme is a simple, quick and efficient method． |
| Key words: talinum crassifolium polysaccharide cellulase orthogonal design |
