| 引用本文: | 李倩，田春芳，刘雯霞，廉宜君，张波，王晓琴，陈韩英.超声波复合酶法提取软紫草多糖的工艺优化[J].中国现代应用药学,2012,29(6):498-503. |
| LI Qian, TIAN Chunfang, LIU Wenxia, LIAN Yijun, ZHANG Bo, WANG Xiaoqin, CHEN Hanying.Optimization of Ultrasonic Pretreatment Followed by Enzymatic Hydrolysis for Extraction Technology of Polysaccharides from Arnebia Euchroma[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(6):498-503. |
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| 摘要: |
| 目的 研究超声波复合酶法提取软紫草多糖的最佳工艺条件。方法 采用单因素分析和正交试验，以软紫草提取物中多糖得率和多糖含量为综合评价指标，考察超声波复合酶法中主要因素对软紫草多糖提取效果的影响。结果 超声波提取优化工艺条件为：超声处理时间40 min，料液比1∶12，功率160 W；在超声波优化结果基础上，进一步进行超声波复合酶法处理，酶解最佳提取条件为酶解时间40 min，酶解温度55 ℃，pH 5.5，纤维素酶、果胶酶、蛋白酶添加量均为2%。结论 超声波复合酶法双重处理可显著提高软紫草多糖的提取效果。 |
| 关键词: 软紫草 多糖 超声波 复合酶 正交试验 |
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| 基金项目:石河子大学自然科学与技术创新重点项目(ZRKX2009ZD01-2) |
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| Optimization of Ultrasonic Pretreatment Followed by Enzymatic Hydrolysis for Extraction Technology of Polysaccharides from Arnebia Euchroma |
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LI Qian, TIAN Chunfang, LIU Wenxia, LIAN Yijun, ZHANG Bo, WANG Xiaoqin, CHEN Hanying1,2
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1.Shihezi University, a.College of Pharmaceutical, b.School of Chemical Engineering, Shihezi 832002, China;2.Key Laboratory of Phytomedicine Resources & Modernization of TCM, Shihezi 832002, China
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| Abstract: |
| OBJECTIVE To study the optimal technological factors for extracting Arnebia euchroma polysaccharide with compound enzymes under ultrasonic．METHODS To develop an optimal procedure based on ultrasonic pretreatment followed by enzymatic hydrolysis for the extraction of polysaccharides from Arnebia euchroma, single factor and orthogonal design methods were used to deal with the effects of crucial ultrasonic pretreatment and hydrolysis parameters on polysaccharide yield. RESULTS The optimal values of ultrasonic power, pretreatment time and material/liquid ratio were determined to be 160 W, 40 min and 1∶12, respectively. The optimization of enzymatic hydrolysis was achieved through the combinatorial use of 2% cellulose, 2% pectinase and 2% neutral proteinase for the hydrolysis for 40 min at pH 5.5 and 55 ℃. CONCLUSION The combination of ultrasonic pretreatment and enzymatic hydrolysis may provide a good idea for the large-scale production of polysaccharides from Arnebia euchroma. |
| Key words: Arnebia euchroma polysaccharide ultrasonic wave compound enzymes orthogonal design |
