| 摘要: |
| 目的 建立西七骨伤片中柚皮苷的含量测定方法。方法 采用高效液相色谱法，色谱柱：Phenomenex Luna C18柱(250 mm×4.6 mm，5 μm)；柱温：30 ℃；流动相：乙腈-0.05%冰醋酸溶液(20∶80)；流速：1.0 mL·min-1；检测波长：283 nm。结果 柚皮苷进样量在0.123 0～1.025 2 μg内与峰面积有良好线性关系，平均加样回收率98.56%，RSD为0.90%(n=6)。结论 该方法简便易行、准确可靠，可用于西七骨伤片的质量控制。 |
| 关键词: 柚皮苷 西七骨伤片 高效液相色谱法 含量测定 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Naringin in Xiqigushang Tablet by HPLC |
|
ZHU Bin, ZHAO Guohua
|
|
Bazhong Institute for Food and Drug Control of Sichuan Province, Bazhong 636000, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for content determination of naringin in Xiqigushang tablet. METHODS The assay was performed on Phenomenex Luna C18 column(250 mm×4.6 mm, 5 μm) by UV detector at 283 nm with acetonitrile-0.05% glacial acetic acid (20∶80) as the mobile phase at the flow rate of 1.0 mL·min-1, column temperature at 30 ℃. RESULTS The amount of naringin was linear with its area over the range of 0.123 0-1.025 2 μg(r=0.999 7), the average recovery and relative standard deviation of naringin were 98.6%, RSD=0.90%(n=6). CONCLUSION The method is sensitive, accurate and can be used for the quality control of Xiqigushang tablet. |
| Key words: naringin Xiqigushang tablet HPLC content determination |
