| 摘要: |
| 目的 利用HPLC同时测定穿心莲药材及其注射液中穿心莲内酯、脱水穿心莲内酯和新穿心莲内酯的含量。方法 色谱柱为Shimadzu Shim-pack VP-ODS柱(4.6 mm×150 mm，5 μm)，以甲醇-水(53∶47)洗脱，流速：1 mL·min-1，柱温：25 ℃，检测波长225 nm处检测穿心莲内酯，检测波长205 nm处检测脱水穿心莲内酯与新穿心莲内酯。结果 穿心莲药材及其注射液中3种内酯类成分能达到很好分离，线性关系良好(r≥0.999 5)；平均加样回收率为99.6%~101.2%，RSD<3%。结论 本方法灵敏、准确、可靠、重复性好，可用于穿心莲药材及其注射液的质量评价与3种内酯类成分的快速测定。 |
| 关键词: 穿心莲 内酯类成分 高效液相色谱法 |
| DOI： |
| 分类号: |
| 基金项目:中央高校基本科研业务费专项资金项目(JKQ2009010) |
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| Simultaneous Determination of 3 Components of Diterpenoids in Andrographis Paniculata and Chuanxinlian Injection by HPLC |
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DONG Haijuan, ZHANG Zunjian
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Center for Instrumental Analysis, China Pharmaceutical University, Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), Nanjing 210009, China
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| Abstract: |
| OBJECTIVE To develop a quantitative method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in Andrographis paniculata and Chuanxinlian injection by HPLC. METHODS A Shimadzu Shim-pack VP-ODS C18 column (4.6 mm×150 mm, 5 μm) was used with CH3OH and water(53∶47) as mobile phase. The flow rate was 1 mL·min-1, the column temperature was 25 ℃, the detection wavelength of andrographolide was set at 225 nm and the detection wavelength of deoxyandrographolide and neoandrographolide was set at 205 nm. RESULTS The 3 active components of diterpenoids were in baseline separation. The linearity of this method was good (r≥0.999 5), and the average recoveries were 99.6%-101.2%, RSD<3%. CONCLUSION The method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Andrographis paniculata and its injection, and for determination of 3 active components of diterpenoids quickly. |
| Key words: Andrographis paniculata diterpenoids HPLC |
