| 引用本文: | 项峥,张剑峰,肖航,窦德强.血塞通注射液致动物急性过敏反应研究[J].中国现代应用药学,2012,29(9):767-771. |
| XIANG Zheng,ZHANG Jianfeng,XIAO Hang,DOU Deqiang.Research in Acute Hypersensitive Reaction Caused by Xuesaitong Injection[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(9):767-771. |
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| 摘要: |
| 目的 探讨血塞通注射液引起的急性过敏反应的机制。方法 采用ELISA法测定给药后豚鼠、小鼠血清中IgE及组胺的含量变化，同时观察其过敏行为的变化，判断急性过敏反应的类型，采用显微观察血塞通注射液引起肥大细胞脱颗粒情况。结果 血塞通注射液给药后引起豚鼠、小鼠的IgE及组胺升高，血塞通注射液引起小鼠的过敏及过敏样反应行为较豚鼠明显。血塞通注射液可引起致敏后及未致敏的肥大细胞脱颗粒。结论 血塞通注射液引起的急性过敏反应为过敏样反应及I型过敏反应，目前中国药典采用的豚鼠评价模型对于评价注射液的过敏样反应有待商榷。 |
| 关键词: 皂苷 急性过敏反应 免疫球蛋白E 组胺 |
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| 基金项目:国家自然科学基金资助项目(30973859)；高等学校博士学科点专项科研基金课题(20092133110001) |
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| Research in Acute Hypersensitive Reaction Caused by Xuesaitong Injection |
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XIANG Zheng, ZHANG Jianfeng, XIAO Hang, DOU Deqiang
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College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116620, China
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| Abstract: |
| OBJECTIVE To study the mechanism of acute hypersensitive reaction caused by Xuesaitong injection. METHODS Contents of IgE and histamine in blood serum of guinea-pig and mouse were determined by kits of ELISA. Anaphylactic behaviors of guinea-pig and mouse were observed after administration. Degranulation of mast cell caused by Xuesaitong injection was observed. RESULTS Contents of IgE and histamine were obviously increased after administration. Manifestations of anaphylactic behaviors of mouse were more obvious. And degranulation of mast cell was observed. CONCLUSION Anaphylaxis and anaphylactoid reaction were the main acute hypersensitive reaction caused by Xuesaitong injection. Guinea-pig was not the experimental animal suitable for assay the anaphylactoid reaction caused by injection. |
| Key words: saponin acute hypersensitive reaction IgE histamine |
