| 引用本文: | 章曙丹，徐强，聂磊.五味子降糖有效部位的HPLC指纹图谱研究[J].中国现代应用药学,2012,29(7):617-620. |
| ZHANG Shudan, XU Qiang, NIE Lei.Study on HPLC Fingerprints of the Antidiabetic-active Fraction of Schisandrae Chinensis Fructus[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(7):617-620. |
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| 摘要: |
| 目的 对五味子降糖有效部位提取物的HPLC指纹图谱进行研究，以更有效地控制产品质量。方法 采用HPLC测定10批样品并建立标准对照指纹图谱。色谱条件：Dikma-C18色谱柱(250 mm×4.6 mm，5 μm)，流动相为甲醇-水，梯度洗脱，流速l mL·min-1，检测波长为 250 nm，柱温为40 ℃。结果 建立了五味子降糖有效部位的 HPLC对照指纹图谱，标示出 9个共有指纹峰。结论 HPLC指纹图谱的建立为科学评价五味子降糖有效部位的质量提供了依据，有助于提高质量控制水平。 |
| 关键词: 五味子 有效部位 指纹图谱 高效液相色谱法 |
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| Study on HPLC Fingerprints of the Antidiabetic-active Fraction of Schisandrae Chinensis Fructus |
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ZHANG Shudan, XU Qiang, NIE Lei1,2
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1.Research Institute of Pharmaceutical Analysis, School of Pharmacy, Shangdong University, Ji’nan 250012, China;2.Research Center of Chinese Traditional Patent Medicine, Zhejiang Research Institute of Traditional Chinese Medicine, Hangzhou 310023, China
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| Abstract: |
| OBJECTIVE To study the HPLC fingerprints of the antidiabetic-active fraction of Schisandrae Chinensis Fructus for better quality control. METHODS Ten samples were analysed to establish the standard fingerprint of the effective parts of Schisandrae Chinensis Fructus by HPLC. The chromatographic conditions were: Dikma-C18(250 mm×4.6 mm, 5 μm) column at the temperature of 40 ℃, a gradient mobile phase of methanol-water with a flow rate of 1 mL·min-1, the detection wavelength was set at 250 nm. RESULTS A standard HPLC fingerprint of the antidiabetic-active fraction of Schisandrae Chinensis Fructus was established with 9 indicated common peaks. CONCLUSION The established fingerprint is reliable and sensitive which could be used in the quality evaluation and control process of Schisandrae Chinensis Fructus products. |
| Key words: Schisandrae chinensis Fructus active fraction chromatographic fingerprint HPLC |
