| 引用本文: | 蔡佳玉，孙树茂，孟永春，南开辉.HPLC测定微球中盐酸左氧氟沙星的含量[J].中国现代应用药学,2012,29(5):437-440. |
| CAI Jiayu, SUN Shumao, MENG Yongchun, NAN Kaihui.Determination of Levofloxacin Hydrochloride in Microspheres by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(5):437-440. |
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| 摘要: |
| 目的 建立测定壳聚糖微球中盐酸左氧氟沙星含量的高效液相色谱法。方法 采用Agilent 1200型高效液相色谱仪，色谱柱为ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5 μm)，柱温：30 ℃，流速：1.0 mL·min-1，流动相：0.04 mol·L-1磷酸溶液(三乙胺调pH值为4)-乙腈(85∶15)，检测波长：293 nm。结果 盐酸左氧氟沙星浓度在1.021~51.15 μg·mL-1内线性关系良好(r=0.999 6)，日内和日间精密度RSD均<2%(n=5)，平均加样回收率为99.1%，RSD为0.72%。结论 本方法简便易行、灵敏度高、重复性好，结果准确可靠，适用于壳聚糖微球中盐酸左氧氟沙星含量测定。 |
| 关键词: 盐酸左氧氟沙星 壳聚糖微球 高效液相色谱法 |
| DOI： |
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| 基金项目:浙江省自然科学基金项目(Y2090914)；浙江省新苗人才计划(2010R413044) |
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| Determination of Levofloxacin Hydrochloride in Microspheres by HPLC |
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CAI Jiayu, SUN Shumao, MENG Yongchun, NAN Kaihui
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Hospital of Optometry & Ophthalmology, Wenzhou Medical College, Wenzhou 325027, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of levofloxacin hydrochloride in chitosan microspheres. METHODS The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm×150 mm, 5 μm) at 30 ℃. The mobile phase was a mixture of 0.04 mol·L-1 phosphoric acid (adjusted to pH 4 with triethylamine) and acetonitrile (85∶15). Flow rate was 1.0 mL·min-1 and UV detection wavelength was 293 nm. RESULTS Good liner relationship was obtained in the range of 1.021-51.15 μg·mL-1(r=0.999 6). Both intra- and inter-day precision RSD were less than 2%(n=5). The average recovery rate was 99.1% with RSD of 0.72%. CONCLUSIONS The established method is sensitive, reliable and suitable for quantitative analysis of levofloxacin hydrochloride in chitosan microspheres. |
| Key words: levofloxacin hydrochloride chitosan microspheres HPLC |
