| 引用本文: | 汪东海，陈敏，姜志强，王萍，陈学锋，徐菊玲.黄芩苷消除鲍曼不动杆菌耐药质粒的实验研究[J].中国现代应用药学,2012,29(5):400-404. |
| WANG Donghai, CHEN Min, JIANG Zhiqiang, WANG Ping, CHEN Xuefeng, XU Juling.Experimental Study of Baicalin Curing Antibiotic-Resistant Plasmid in Acinetobacter Baumanii[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(5):400-404. |
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| 摘要: |
| 目的 探讨黄芩苷能否消除鲍曼不动杆菌质粒，并影响抗菌药物对细菌的最低抑菌浓度(MIC)。方法 测定黄芩苷处理的鲍曼不动杆菌生长曲线。16株庆大霉素和环丙沙星双重耐药的鲍曼不动杆菌，经黄芩苷作用20 h，分别在处理前和处理后应用碱裂解法检测质粒，琼脂稀释法测定抗菌药物MIC。结果 黄芩苷能抑制鲍曼不动杆菌生长。16株鲍曼不动杆菌耐药株均携带质粒，经1 mg·mL-1黄芩苷作用后，质粒消除率为37.5%(6/16)。6株经黄芩苷消除质粒的细菌，庆大霉素中度水平或低水平耐药株的MIC值均下降至敏感水平；环丙沙星低水平耐药株的MIC值下降至敏感水平，部分环丙沙星中度水平耐药株的MIC值下降但高于耐药水平。结论 黄芩苷能够消除鲍曼不动杆菌低水平耐药株携带的质粒，恢复菌株对庆大霉素和环丙沙星的敏感性，可用于鲍曼不动杆菌低水平耐药株感染辅助治疗。 |
| 关键词: 黄芩苷 质粒 鲍曼不动杆菌 庆大霉素 环丙沙星 |
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| 基金项目:湖州市科技计划项目(2010YSB04) |
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| Experimental Study of Baicalin Curing Antibiotic-Resistant Plasmid in Acinetobacter Baumanii |
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WANG Donghai, CHEN Min, JIANG Zhiqiang, WANG Ping, CHEN Xuefeng, XU Juling1,2
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1.Zhejiang Province Huzhou First People's Hospital, Huzhou 313000, China;2.Medical School of Huzhou Teachers College, Huzhou 313000, China
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| Abstract: |
| OBJECTIVE To investigate if baicalin was able to cure antibiotic-resistant plasmid in Acinetobacter baumanii, and affect minimum inhibitory concentrations (MIC) of antibiotics to bacteria. METHODS The growth curve of A. baumanii treated with baicalin was detected. 16 strains of A. baumanii, gentamicin(GEN) and ciprofloxacin(CIP) resistance, were treated with baicalin for 20 hours. Before and after baicalin treatment, plasmids of A. baumanii were determined with alkaline lysis method, and MICs of GEN and CIP were measured by agar dilution method. RESULTS Baicalin inhibited the growth of A. baumanii. The plasmid curing rate was 37.5%(6/16) in 16 strains of A. baumanii with plasmids, after the treatment with baicalin (1 mg.mL-1). In 6 strains of A. baumanii with plasmid cured by baicalin, for medium or low level of GEN-resistance strains, MICs of GEN all decreased to sensitive level; for low level of CIP-resistance strains, MICs of CIP all dropped to sensitive level, and for some medium-level of CIP-resistance strains, MICs of CIP also dropped, but still above the resistance level. CONCLUSION Baicalin can eliminate plasmids of A. baumanii with low level of antibiotic-resistance, and make these strains recover the susceptibility to GEN and CIP, which shows an adjuvant treatment method for antibiotic-resistant A. baumanii infections. |
| Key words: baicalin plasmid Acinetobacter baumannii gentamicin ciprofloxacin |
