摘要: |
目的 确定唐古特红景天药材的最佳提取工艺。方法 采用超声、回流、冷浸法,采用L9(34)正交试验法,考察提取温度、乙醇浓度、溶剂量、提取时间4因素对红景天苷、酪醇含量和提取率的影响。结果 回流提取为最适提取方法,最佳提取工艺条件为:8倍量50%乙醇80 ℃提取2次,每次提取1.0 h。结论 该研究结果为有效利用唐古特红景天资源及其工业生产提供了依据。 |
关键词: 唐古特红景天 红景天苷 酪醇 正交试验 |
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基金项目:“十一五”国家科技重大专项(2009ZX09501-015) |
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Study on the Optimized Extraction Technology of Rhodiola Algida |
HE Meiyan, ZHANG Xiaolong1,2
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1.Sanjin Group Hunan Sanjin Pharmaceutical Co. Ltd., Changde 415001, China;2.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
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Abstract: |
OBJECTIVE To optimize the extraction technology of Rhodiola algida. METHODS Rhodiola algida was extracted by ultrasonic, refluxing and cold soaking extraction, respectively. The extraction rate, content of salidroside and p-tyrosol were chosen as the assessment index by using the orthogonal design. The effects of ethanol content, solvent volume, extracting time (hour) and extraction temperature were investigated. RESULTS The results indicated that the refluxing extraction was the best method. The optimized extraction process conditions were as follows: adding 8 fold of 50% ethanol, extracting for 1.0 h and 2 times at 80 ℃. CONCLUSION This study will provide a reference to the research of Rhodiola algida and industrial production. |
Key words: Rhodiola algida salidroside p-tyrosol orthogonal test |