| 引用本文: | 蔡银燕，石婷婷，黄巧玲.HPLC测定六味地黄浓缩丸中5-HMF的含量[J].中国现代应用药学,2012,29(4):356-358. |
| CAI Yinyan, SHI Tingting, HUANG Qiaoling.Determination of 5-Hydroxymethylfurfural in Liuweidihuang Pills by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(4):356-358. |
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| 摘要: |
| 目的 建立六味地黄浓缩丸中5-羟甲基糠醛(5-HMF)的含量测定方法。方法 采用Agilent Zorbax C18色谱柱 (250 mm×4. 6 mm，5 μm)；流动相为甲醇-水(20∶80)；流速：1.0 mL·min-1；检测波长：280 nm；柱温：30 ℃。结果 5-HMF在0.026 2~2.62 mg·mL-1内线性关系良好，平均回收率为99.7%，RSD为0.57%。结论 该方法简便、快速、准确度好，可有效控制该制剂中5-羟甲基糠醛含量。 |
| 关键词: 六味地黄浓缩丸 5-羟甲基糠醛 高效液相色谱法 含量测定 |
| DOI： |
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| 基金项目:国家自然科学基金（No. 30873438） |
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| Determination of 5-Hydroxymethylfurfural in Liuweidihuang Pills by HPLC |
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CAI Yinyan, SHI Tingting, HUANG Qiaoling
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Hangzhou No.3 People’s Hospital, Hangzhou 310009, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of 5-hydroxymethylfurfural(5-HMF) in Liuweidihuang pills. METHODS The HPLC method was performed on an Agilent Zorbax C18 column(250 mm×4.6 mm, 5 μm) with 284 nm as the detection wavelength, and methanol-water(20∶80) was used as mobile phase. The column temperature was 30 ℃, and the flow rate was 1.0 mL·min-1. RESULTS The regression results which was based on weighted least square method showed a good linearity within the range of 0.026 2-2.62 mg·mL-1, and the average recovery was 99.7% with RSD of 0.57%. CONCLUSION The HPLC method was accurate, sensitive and suitable for determining the content of 5-HMF in Liuweidihuang pills. |
| Key words: Liuweidihuang pills 5-HMF HPLC content determination |
