| 引用本文: | 董敬远，任亮，陶志敏，张素贞，崔春蕾.灯盏花素对梗阻性肾病大鼠肾间质及血管活性物质的影响[J].中国现代应用药学,2012,29(2):109-112. |
| DONG Jingyuan, REN Liang, TAO Zhimin, ZHANG Suzhen, CUI Chunlei.Role of Breviscapine on Renal Interstitial and Vascular Active Matter in Rats with Obstructive Nephropathy[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(2):109-112. |
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| 摘要: |
| 目的 研究灯盏花素对梗阻性肾病大鼠肾间质及血管活性物质的影响。方法 将SD大鼠随机分为假手术组、模型组和灯盏花素组。灯盏花素组给予灯盏花素200 mg·kg-1灌胃，假手术组及模型组则给予0.5%羧甲基纤维素钠溶液灌胃，每天1次，连续7 d。HE及Masson染色观察大鼠肾组织病理改变，检测肾脏重量变化，血液及肾组织中转化生长因子β1(TGF-β1)及内皮素(ET-1)和一氧化氮(NO)的含量。结果 模型组大鼠左侧肾脏肾间质可见大量炎性细胞浸润及充血，且重量与假手术组比较显著增加(P<0.01)。模型组大鼠血清中TGF-β1及NO、ET-1含量与假手术组相比存在明显差异(P<0.01)。两侧肾组织中TGF-β1及NO、ET-1 含量分别与假手术组存在明显差异(P<0.01或P<0.001)。TGF-β1和ET-1 含量升高而NO含量降低。而灯盏花素组大鼠肾间质损伤明显改善，左侧肾脏重量下降(P<0.05)，血清中TGF-β1及NO、ET-1含量、肾组织中TGF-β1及NO、ET-1 含量分别与模型组存在明显差异(P<0.05或P<0.01)，TGF-β1及ET-1 含量降低而NO 升高。结论 灯盏花素可通过下调血液及肾组织中TGF-β1和ET-1含量、上调NO浓度等作用减轻梗阻性肾病肾间质病变。 |
| 关键词: 灯盏花素 梗阻性肾病 肾间质 转化生长因子β1 血管活性物质 |
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| Role of Breviscapine on Renal Interstitial and Vascular Active Matter in Rats with Obstructive Nephropathy |
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DONG Jingyuan, REN Liang, TAO Zhimin, ZHANG Suzhen, CUI Chunlei1,2
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1.Luohe Central Hospital, the First Affiliated Hospital of Luohe Medical College, Luohe 462001, China;2.Luohe Medical College, Luohe 462002, China
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| Abstract: |
| OBJECTIVE To observe and analyse the role of breviscapine on renal interstitial and vascular active matter in rats with obstructive nephropathy. METHODS The rats were randomly divided into three groups: sham group(SG), model group (MG) and breviscapine group(BG). Left ureters in MG and BG were ligated and then reproduced obstructive nephropathy model, while in SG the ureter was isolated only without further manipulation. The rats in BG were given breviscapine treatment(200 mg·kg-1), SG and MG were given 0.5% CMC-Na once a day for seven days by gavage. The pathological changes in renal interstitial were observed by HE staining, changes in their serum level and renal tissue of TGF-β1, ET-1 and NO were determined. RESULTS There was serious hyperremia in renal interstitial, inflammatory cells infiltration in MG, also the left renals were heavier than those in SG(P<0.01). The contents of TGF-β1 and ET-1 in MG were increased but the activity of NO were declined in serum level than that in SG(P<0.01), also the contents of TGF-β1 and ET-1 in MG were increased but the activity of NO was declined in renal tissue than that in SG(P<0.01, P<0.001). Hyperremia, infiltration, broking were significantly improved in BG. The left renals in BG were lighter than those in MG(P<0.05). The contents of TGF-β1 and ET-1 in BG were declined but the activity of NO was increased in serum level than that in MG(P<0.05), also the contents of TGF-β1 and ET-1 in BG were declined but the activity of NO was increased in renal tissue than that in MG(P<0.05, P<0.01). CONCLUSION The changes of TGF-β1, ET-1 and NO may be significant correlated with renal interstitial in obstructive nephropathy. Also, the renal interstitial in obstructive nephropathy may be alleviated by breviscapine through down-regulating the TGF-β1 in serum level and renal tissue, and protecting the vascular active matter, thus inhibitting the procedure of renal interstitial fibrosis. |
| Key words: breviscapine obstructive nephropathy renal interstitial TGF-β1 vascular active matter |
