| 引用本文: | 冯连晶,陈筱瑜,黄庆德,陈丹.星点设计-响应面法优化石杉碱甲二元醇质体处方及其理化性质考察[J].中国现代应用药学,2022,39(2):181-186. |
| FENG Lianjing,CHEN Xiaoyu,HUANG Qingde,CHEN Dan.Optimization of the Formulation of Huperzine A Binary Ethosomes by Central Composite Design-response Surface Methodology and Its Physicochemical Properties[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(2):181-186. |
|
| 摘要: |
| 目的 优化石杉碱甲二元醇质体的处方，并对其理化性质进行考察。方法 采用注入法制备石杉碱甲二元醇质体。以卵磷脂含量(X1)、无水乙醇含量(X2)、混合醇含量(X3)为考察因素，以包封率(Y1)与平均粒径(Y2)为评价指标，通过星点设计-响应面法优化醇质体处方。并考察醇质体的粒径、电位等理化性质。结果 优选的处方为卵磷脂含量3.60%，无水乙醇含量22.50%，混合醇含量45.00%，在此条件下，石杉碱甲二元醇质体的平均包封率为(94.05±0.22)%，平均粒径为(260.1±5.7)nm，Zeta电位为(-20.9±0.3)mV，PDI值为0.188±0.056。结论 星点设计-响应面法建立的响应方程具有较好的预测性，可用于石杉碱二元醇质体处方的优化，所得二元醇质体性质稳定。 |
| 关键词: 石杉碱甲 二元醇质体 处方优化 星点设计-响应面法 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.02.007 |
| 分类号:R283.6 |
| 基金项目:福建省科技厅引导性项目(2018Y0050) |
|
| Optimization of the Formulation of Huperzine A Binary Ethosomes by Central Composite Design-response Surface Methodology and Its Physicochemical Properties |
|
FENG Lianjing1, CHEN Xiaoyu2, HUANG Qingde1, CHEN Dan1
|
|
1.School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;2.Department of Pharmacy, Fujian Health College, Fuzhou 350101, China
|
| Abstract: |
| OBJECTIVE To optimize the formulation of huperzine A binary ethosomes and investigate its physicochemical properties. METHODS The injection method was used to prepare huperzine A binary ethosomes. Taking lecithin content(X1), absolute ethanol content(X2) and mixed alcohol content(X3) as the inspection factors, central composite design-response surface method and overall desirability were used to optimize the formulation with the encapsulation efficiency(Y1) and average particle size(Y2) as evaluation indicators. The physicochemical properties of ethosomes such as particle size and potential were investigated. RESULTS The optimum preparation of formulation were the lecithin content of 3.60%, the absolute ethanol content of 22.50% and the mixed alcohol content of 45.00%. Under these conditions, the average encapsulation efficiency was (94.05±0.22)%, the average particle size was (260.1±5.7)nm, the Zeta potential was (-20.9±0.3)mV and the PDI was 0.188±0.056. CONCLUSION The response equation established by central composite design-response surface method has good predictability, and can be used to optimize the formulation of huperzine A binary ethosomes. The obtained ethosomes have stable properties. |
| Key words: huperzine A binary ehosomes prescription optimization central composite design-response surface method |
