| 引用本文: | 朱丽娟,罗建云,宋润泽,王丽娟,张安平,臧凯宏.当归挥发油通过抑制PI3K/Akt/mTOR信号转导通路影响人结肠癌LOVO细胞自噬研究[J].中国现代应用药学,2022,39(4):437-441. |
| ZHU Lijuan,LUO Jianyun,SONG Runze,WANG Lijuan,ZHANG Anping,ZANG Kaihong.Study on Volatile Oil of Angelicae Sinensis Radix Affects Autophagy in Human Colorectal Cancer LOVO Cells by Inhibiting PI3K/Akt/mTOR Signal Transduction Pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(4):437-441. |
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| 当归挥发油通过抑制PI3K/Akt/mTOR信号转导通路影响人结肠癌LOVO细胞自噬研究 |
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朱丽娟1,2, 罗建云3, 宋润泽4, 王丽娟1,2, 张安平4, 臧凯宏1,2
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1.甘肃中医药大学药学院, 兰州 730000;2.甘肃省中药药理与毒理学重点实验室, 兰州 730000;3.陕西省西安市医疗保障局, 西安 710021;4.兰州大学第二医院血管外科, 兰州 730000
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| 摘要: |
| 目的 研究当归挥发油(volatile oil of Angelicae Sinensis Radix,VOAS)对人结直肠癌LOVO细胞自噬的影响及其分子机制。方法 用不同浓度的VOAS (6.25,12.5,25,50和100 μg·mL-1)作用于人结肠癌LOVO细胞48 h,CCK8比色法检测VOAS对LOVO细胞增殖的抑制作用。将VOAS 50 μg·mL-1作用于LOVO细胞,AO荧光染色和透射电镜观察细胞自噬情况;Western blotting观察VOAS对自噬相关蛋白LC3B、Beclin-1、Atg5及PI3K/Akt/mTOR信号转导通路的影响。结果 6.25~100 μg·mL-1的VOAS能够抑制LOVO细胞的增殖,具有浓度依赖性;50 μg·mL-1的VOAS能够促进LOVO细胞的自噬,上调自噬相关蛋白LC3B-Ⅱ、Beclin-1及Atg5的表达,同时下调PI3K、p-Akt、p-mTOR蛋白的表达。结论 VOAS能够促进人结肠癌LOVO细胞自噬,其机制可能与抑制PI3K/Akt/mTOR信号转导通路有关。 |
| 关键词: 当归挥发油 LOVO 细胞 自噬 PI3K/Akt/mTOR 信号转导通路 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.04.002 |
| 分类号:R285.5 |
| 基金项目:甘肃省高等学校科研基金资助项目(2018-A050)；甘肃省中药药理与毒理学重点实验室开放基金资助项目(ZDSYS-KJ-2018-008) |
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| Study on Volatile Oil of Angelicae Sinensis Radix Affects Autophagy in Human Colorectal Cancer LOVO Cells by Inhibiting PI3K/Akt/mTOR Signal Transduction Pathway |
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ZHU Lijuan1,2, LUO Jianyun3, SONG Runze4, WANG Lijuan1,2, ZHANG Anping4, ZANG Kaihong1,2
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1.College ofPharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China;2.Gansu Province Key Laboratory of TCM Pharmacology and Toxicology, Lanzhou 730000, China;3.Xi'an Healthcare Security Bureau of Shaanxi Province, Xi'an 710021, China;4.Department of Vascular Surgery, Lanzhou University Second Hospital, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To study the effect and mechanism of volatile oil of Angelicae Sinensis Radix(VOAS) on autophagy in human colorectal cancer LOVO cells. METHODS Human colorectal cancer LOVO cells were treated with various concentrations of VOAS(6.25, 12.5, 25, 50 and 100 μg·mL-1) for 48 h, CCK8 assay was used to detect the effect of VOAS on the proliferation inhibition effect of cells. When treated with VOAS(50 μg·mL-1), the effect of VOAS on autophagy was observed by acridine orange staining and electron microscopy, Western blotting was used to detect the expression of autophagy hallmark protein(LC3B, Beclin-1, Atg5) and PI3K/Akt/mTOR signal pathway. RESULTS The 6.25-100 μg·mL-1 VOAS could significantly inhibit the proliferation of LOVO cells in dose-dependent manner, and VOAS(50 μg·mL-1) could promote autophagy. Furthermore, VOAS(50 μg·mL-1) could up-regulate the expression of LC3B-II, Beclin-1 and Atg5 protein that related to autophagy, and down-regulate the expression of PI3K, p-Akt and p-mTOR protein. CONCLUSION VOAS may induce autophagy in human colorectal cancer LOVO cells through inhibiting PI3K/Akt/mTOR signal transduction pathway. |
| Key words: volatile oil of Angelicae Sinensis Radix LOVO cells autophagy PI3K/Akt/mTOR pathway |
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