| 引用本文: | 李洋洋,王磊,张利英,张苡铭,何进鹏,周婷,周谷城,牛帆,刘永琦.黄芪多糖对X射线辐射所致BMSCs全基因组低甲基化的防护作用[J].中国现代应用药学,2022,39(10):1261-1267. |
| LI Yang-yang,WANG Lei,ZHANG Li-ying,ZHANG Yi-ming,HE Jin-peng,ZHOU Ting,ZHOU Gu-cheng,NIU Fan,LIU Yong-qi.Protective Effect of Astragalus Polysaccharide on the Hypomethylation of BMSCs Genome Induced by Multiple X-ray Radiation[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(10):1261-1267. |
|
| |
|
|
| 本文已被：浏览 882次 下载 475次 |
码上扫一扫！ |
|
|
| 黄芪多糖对X射线辐射所致BMSCs全基因组低甲基化的防护作用 |
|
李洋洋1, 王磊2, 张利英1, 张苡铭1, 何进鹏3, 周婷1, 周谷城1, 牛帆1, 刘永琦1,4
|
|
1.甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000;2.中国中医科学院西苑医院, 北京 100091;3.甘肃省空间辐射生物学重点实验室中国科学院近代物理研究所, 兰州 730000;4.敦煌医学与转化教育部重点实验室, 兰州 730000
|
|
| 摘要: |
| 目的 研究黄芪多糖(Astragalus polysaccharide,APS)对多次X线电离辐射(ionizing radiation,IR)所致骨髓间充质干细胞(bone marrow stem cells,BMSCs)全基因组甲基化水平的影响。方法 采用APS作用于5次每次2 Gy X射线辐射的BMSCs,将BMSCs分为对照组、APS组、IR组和APS+IR组。采用胞质分裂阻断法测定微核形成率;细胞染色体核型分析检测细胞核损伤;EPIGENTEK试剂盒检测各组BMSCs全基因组甲基化程度;Westernblotting检测各组BMSCs甲基转移酶DNMT3a、DNMT3b,甲基化CpG结合蛋白2(MeCP2)、甲基化CpG结合域蛋白2(MBD2)和各组BMSCs癌基因c-Myc、H-ras,抑癌基因P53、P16的蛋白表达水平。结果 与对照组比较,IR组微核数目和染色体畸变明显增加(P<0.01),BMSCs全基因组甲基化程度明显降低(P<0.01);与IR组比较,APS+IR组微核数目和染色体畸变减少(P<0.05),BMSCs全基因组甲基化程度升高(P<0.01)。在蛋白表达上,与对照组比较,IR组DNMT3a和MeCP2均明显降低(P<0.01),c-Myc、H-ras的表达明显升高,P53、P16的表达显著降低(P<0.01);与IR组比较,APS+IR组DNMT3a和MeCP2明显升高(P<0.01或P<0.05),c-Myc、H-ras的表达降低(P<0.01),P53、P16的表达显著升高(P<0.01)。结论 黄芪多糖能够防治多次X线辐射所致BMSCs全基因组甲基化水平,降低BMSCs的恶化风险。 |
| 关键词: 黄芪多糖 电离辐射 骨髓间充质干细胞 全基因组甲基化 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.10.001 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目(81473457,81973595);甘肃省高等学校科研项目(2015A-095);西北中藏药协同创新中心项目(XBXT2015-1);甘肃省中医药研究中心开放基金项目(zyzx-2020-18);甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室开放基金项目(FZYX17-18-13);敦煌医学与转化教育部重点实验室开放基金项目(DHYX19-13) |
|
| Protective Effect of Astragalus Polysaccharide on the Hypomethylation of BMSCs Genome Induced by Multiple X-ray Radiation |
|
LI Yang-yang1, WANG Lei2, ZHANG Li-ying1, ZHANG Yi-ming1, HE Jin-peng3, ZHOU Ting1, ZHOU Gu-cheng1, NIU Fan1, LIU Yong-qi1,4
|
|
1.Gansu University of Traditional Chinese Medicine, Provincial Key Laboratory of Molecular Medicine for Critical Diseases and Prevention and Treatment of Traditional Chinese Medicine in Universities of Gansu Province, Lanzhou 730000, China;2.Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;3.Key Laboratory of Space Radiation Biology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;4.The Key Laboratory of Dunhuang Medicine and Transformation of the Ministry of Education, Lanzhou 730000, China
|
| Abstract: |
| OBJECTIVE To study the effect of Astragalus polysaccharide(APS) on the whole genome methylation level of bone marrow stem cells(BMSCs) induced by multiple X-ray ionizing radiation(IR).METHODS APS was used to act on BMSCs irradiated 5 times with 2 Gy X-rays per time, and BMSCs were divided into control group, APS group, IR group and APS+IR group. Cytokinesis blocking method was used to determine the rate of micronucleus formation; cell chromosome karyotype analysis was used to detect nuclear damage; EPIGENTEK kit was used to detect the degree of methylation of the whole genome of BMSCs in each group; Western blotting was used to detect the expression levels of BMSCs methyltransferase DNMT3a, DNMT3b, methyl-Cp G-binding protein 2(MeCP2), methyl-Cp G-binding domain protein 2(MBD2), oncogenes c-Myc, H-ras, and tumor suppressor genes P53 and P16 of BMSCs in each group.RESULTS Compared with the control group, the number of micronuclei and chromosome aberrations in IR group increased significantly(P<0.01), the degree of methylation of the whole genome of BMSCs in the IR group was significantly decreased(P<0.01); compared with IR group, the number of micronuclei and chromosome aberrations in the APS+IR group decreased(P<0.05), the degree of methylation of the whole genome of BMSCs in APS+IR group increased(P<0.01). In terms of protein characterization, compared with the control group, DNMT3a and MeCP2 in IR group were significantly decreased(P<0.01), the expressions of c-Myc and H-ras were significantly increased, and the expressions of P53 and P16 were significantly decreased(P<0.01). Compared with IR group, DNMT3a and MeCP2 in the APS+IR group were significantly increased(P<0.01 or P<0.05), the expressions of c-Myc and H-ras were decreased(P<0.01), and the expressions of P53 and P16 were significantly increased(P<0.01).CONCLUSION APS can prevent and control the methylation level of the whole genome of BMSCs induced by multiple X-ray radiation, and reduce the risk of deterioration of BMSCs. |
| Key words: Astragalus polysaccharide ionizing radiation bone marrow mesenchymal stem cells whole genome methylation |
|
|
|
|
