| 引用本文: | 钟飞,李梦婷,杜娅娅,许正新.三七皂苷Fc对SD大鼠心室肌细胞钠离子通道电流的影响[J].中国现代应用药学,2022,39(9):1162-1167. |
| ZHONG Fei,LI Mengting,DU Yaya,XU Zhengxin.Effects of Notoginsenoside Fc on Sodium Ion Channel Currents in Ventricular Myocytes of SD Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(9):1162-1167. |
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| 三七皂苷Fc对SD大鼠心室肌细胞钠离子通道电流的影响 |
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钟飞1, 李梦婷1, 杜娅娅1, 许正新1,2,3,4
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1.扬州大学医学院药学系, 江苏 扬州 225000;2.江苏省重点动物传染病和人畜共患病预防控制共创中心, 江苏 扬州 225001;3.江苏省中西医结合老年病防治重点实验室, 江苏 扬州 225001;4.江苏省非编码RNA基础与临床转化重点实验室, 江苏 扬州 225009
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| 摘要: |
| 目的 探究三七皂苷Fc (notoginsenoside Fc，N-Fc)对SD大鼠心室肌细胞钠离子通道电流的影响。方法 使用Langendorff恒温恒压灌流装置通过酶解法急性分离SD大鼠心室肌细胞，采用标准全细胞膜片钳技术观察并记录不同浓度的N-Fc对大鼠心室肌细胞钠通道电流(INa)及其动力学特征的影响。结果 5µmol·L–1的N-Fc对INa无明显影响，随着药物浓度的增加，10，20，50µmol·L–1的N-Fc可使钠峰值电流由给药前的(–87.49±3.40) pA/pF依次下降为(–62.91±2.37)，(–49.30±1.27)，(–34.68±3.21) pA/pF (P<0.01)；INa的I-V曲线上移，但形态轨迹、激活电位及峰电位基本不变；INa的激活曲线向去极化方向迁移，半数激活电压由给药前的(–52.37 ±1.32) mV变为(–42.74 ±0.37)，(–38.92 ±2.77)，(–33.78 ±0.96) mV (P<0.05)；失活曲线显著左移，半数失活电压(V1/2-in)由(–55.95±4.88) mV依次变为(–64.29 ±1.77)，(–69.57±3.47)，(–73.32±3.79) mV (P<0.05)；此外，N-Fc能够显著延长INa失活后恢复时间，时间常数τ值(给药前，10，20，50µmol·L–1N-Fc处理后)分别为(12.50±1.17)，(21.36±2.23)，(25.35±1.25)，(34.35±1.24) ms (P<0.05)。结论 N-Fc能浓度依赖性地抑制SD大鼠心室肌细胞的INa，并能显著影响其激活、失活及失活后恢复的动力学特征。 |
| 关键词: 三七皂苷Fc 膜片钳 钠通道 心室肌细胞 SD大鼠 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.09.005 |
| 分类号:R965.2 |
| 基金项目:扬州大学大学生科研创新计划项目(X20200782) |
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| Effects of Notoginsenoside Fc on Sodium Ion Channel Currents in Ventricular Myocytes of SD Rats |
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ZHONG Fei1, LI Mengting1, DU Yaya1, XU Zhengxin1,2,3,4
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1.Department of Pharmacology, School of Medicine, Yangzhou University, Yangzhou 225000, China;2.Key Joint Center of Infectious Diseases and Zoonosis Prevention and Control of Jiangsu Province, Yangzhou 225001, China;3.Key Laboratory of Integrative Medicine in Geriatrics Control of Jiangsu Province, Yangzhou 225001, China;4.Key Laboratory of Experimental & Translational Non-coding RNA Research of Jiangsu Province, Yangzhou 225009, China
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| Abstract: |
| OBJECTIVE To explore the effects of notoginsenoside Fc(N-Fc) on sodium ion channel currents of ventricular myocytes in SD rats. METHODS Ventricular myocardial cells were enzymatically isolated from SD rats by Langendorff with constant pressure and temperature, the whole-cell patch clamp techniques were used to observe and record the effects of INa and its kinetics in ventricular mgocytes of SD rats treated with different concentrations of N-Fc. RESULTS There was no significant effect on INa when the concentration of N-Fc was 5 µmol·L–1, with the increase of drug concentration, the peak sodium current was decreased from (–87.49±3.40)pA/pF to (–62.91±2.37), (–49.30±1.27), (–34.68±3.21)pA/pF(P<0.01) under the effects of 10, 20 and 50 µmol·L–1 N-Fc. I-V curve of INa were shifted upward, but the morphological trajectories, activation potential and peak potential did not change. The activation curve of INa were shifted to the depolarization direction, and the half in activation voltage was changed from (–52.37 ±1.32)mV to (–42.74 ±0.37), (–38.92 ±2.77), (–33.78 ±0.96)mV(P<0.05). The inactivation curve shifted significantly to the left, the half inactivation voltage changed from (–55.95±4.88)mV to (–64.29±1.77), (–69.57±3.47), (–73.32±3.79)mV(P<0.05). In addition, N-Fc significantly prolonged the recovery time after INa inactivation, and the time constant τ(before administration, after treated with 10, 20, 50 µmol·L -1N-Fc) were (12.50±1.17), (21.36±2.23), (25.35±1.25), (34.35±1.24)ms(P<0.05). CONCLUSION N-Fc can inhibit INa of SD rats ventricular myocytes in a concentration-dependent manner and it has an significant effect on the kinetics characteristics of activation, inactivation and recovery. |
| Key words: notoginsenoside Fc patch clamp sodium channel ventricular myocytes SD rats |
