| 引用本文: | 杜正彩,俞航萍,陈章美,夏中尚,赵芳惠,梁云飞,徐懿乔,郝二伟,侯小涛,邓家刚.中华跌打丸对斑马鱼骨折愈合作用研究[J].中国现代应用药学,2021,38(12):1454-1460. |
| DU Zhengcai,YU Hangping,CHEN Zhangmei,XIA Zhongshang,ZHAO Fanghui,LIANG Yunfei,XU Yiqiao,HAO Erwei,HOU Xiaotao,DENG Jiagang.Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(12):1454-1460. |
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| 中华跌打丸对斑马鱼骨折愈合作用研究 |
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杜正彩1, 俞航萍2, 陈章美1, 夏中尚3, 赵芳惠1, 梁云飞4, 徐懿乔2, 郝二伟5, 侯小涛5, 邓家刚5
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1.广西中医药大学, 科学实验中心, 南宁 530200;2.杭州环特生物科技股份有限公司, 杭州 310051;3.成都中医药大学, 成都 611137;4.广西梧州制药(集团)股份有限公司, 广西 梧州 543000;5.广西中医药大学, 广西中药药效研究重点实验室, 南宁 530200
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| 摘要: |
| 目的 应用斑马鱼研究中华跌打丸促骨折愈合、抗炎、促血管形成及软骨损伤修复作用。方法 采用顿性棱刀挤压斑马鱼尾鳍建立骨折模型，评价其骨折愈合及抗炎作用；用转基因血管荧光斑马鱼评价其促血管形成作用；地塞米松建立斑马鱼软骨损伤模型，评价其软骨损伤修复作用；通过Q-PCR检测中华跌打丸对TNF-α、IL-1β、BMP-2和BMP-7基因表达的影响。结果 与模型对照组比较，中华跌打丸在浓度为每尾2 mg时，骨折愈合促进作用为20.8%（P<0.05）；浓度在15.6，31.3和62.5 mg·mL-1时炎症消退作用分别为44.6%，50.6%和52.6%（P<0.05），且伴随TNF-α和IL-1β基因的表达有不同程度下调（P<0.05或P<0.01）；浓度为1 000，2 000 mg·L-1时，促血管形成作用分别为19.7%和49.9%（P<0.01或P<0.001）；中华跌打丸通过上调BMP-2和BMP-7基因的表达起到软骨修复作用（P<0.01或P<0.001），浓度为500 mg·L-1时，软骨损伤修复作用为42.0%（P<0.05）。结论 中华跌打丸对斑马鱼有促进骨折愈合、抗炎、促血管及软骨损伤修复作用，且通过下调TNF-α和IL-1β的表达起到消炎作用，上调BMP-2和BMP-7基因的表达起到软骨修复作用。 |
| 关键词: 中华跌打丸|斑马鱼|骨折愈合|抗炎|促血管形成|软骨损伤修复 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.12.008 |
| 分类号:R285.5 |
| 基金项目:广西科技重大专项（桂科AA17202040-2）；广西中医药大学校级重点硕士研究生科研创新项目（YCSZ20190022）；广西中药药效研究重点实验室项目（19-050-39）；广西农作物废弃物功能成分研究协同创新中心建设项目 |
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| Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish |
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DU Zhengcai1, YU Hangping2, CHEN Zhangmei1, XIA Zhongshang3, ZHAO Fanghui1, LIANG Yunfei4, XU Yiqiao2, HAO Erwei5, HOU Xiaotao5, DENG Jiagang5
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1.Guangxi University of Traditional Chinese Medicine, Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Nanning, 530200, China;2.Hunter Biotechnology, Co., Ltd, Hangzhou 310051, China;3.Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;4.Guangxi Wuzhou Pharmaceutical (Group) Co., Ltd, Wuzhou, 543000, China;5.Guangxi University of Traditional Chinese Medicine, Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning, 530200, China
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| Abstract: |
| OBJECTIVE To study the antifracture healing, anti-inflammatory, angiogenesis and repair of cartilage damage effects of Zhonghua Dieda pill by using zebrafish. METHODS The fracture model was established by squeezing the tail fin of zebrafish by a blunt edge knife, and the effect of Zhonghua Dieda pill on fracture healing and anti-inflammatory was then evaluated; transgenic vascular fluorescence zebrafish was used to evaluate the angiogenic effect; cartilage damage model was established by treating zebrafish with dexamethasone, and then the effect of cartilage damage reparing was evaluated; the effects of Zhonghua Dieda pill on the gene expressions of TNF-α, IL-1β, BMP-2 and BMP-7 were measured by Q-PCR. RESULTS Compared with the model control group, the fracture healing effect of the Zhonghua Dieda pill was 20.8%(P<0.05) when concentration was 2 mg per tail. With down-regulating the expression of TNF-α and IL-1β genes(P<0.05 or P<0.01), the anti-inflammatory effect of Zhonghua Dieda pill was 44.6%, 50.6% and 52.6% at the 15.6, 31.3 and 62.5 mg·mL-1 concentration(P<0.05); the angiogenesis effect of Zhonghua Dieda pill was 19.7% and 49.9% at the 1 000, 2 000 mg·L-1 concentration groups(P<0.01 or P<0.001); Zhonghua Dieda pill showed cartilage damage repairing effect by up-regulating the expression of BMP-2 and BMP-7 genes and result in 42.0% effect of cartilage damage repairing on zebrafish model at 500 mg·mL-1 concentration(P<0.05). CONCLUSION Zhonghua Dieda pill can promote fracture healing, anti-inflammatory, angiogenesis and cartilage damage repairing in zebrafish, and play an anti-inflammatory role by down-regulating the expression of TNF-α and IL-1β and play a cartilage repairing by up-regulating the expression of BMP-2 and BMP-7 genes. |
| Key words: Zhonghua Dieda pill|zebrafish|fracture healing|anti-inflammatory|angiogenesis |
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