| 引用本文: | 董亚娜,郭宝中,侯晶晶,袁振英,唐亚辉,杨娟.松果菊苷抑制卵巢癌SKOV3细胞的增殖、侵袭和干细胞样特性[J].中国现代应用药学,2022,39(10):1289-1295. |
| DONG Ya-na,GUO Bao-zhong,HOU Jing-jing,YUAN Zhen-ying,TANG Ya-hui,YANG Juan.Echinacoside Inhibits the Proliferation, Invasion and Stem Cell-like Properties of Ovarian Cancer SKOV3 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(10):1289-1295. |
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| 摘要: |
| 目的 探讨松果菊苷对卵巢癌SKOV3细胞的作用。方法 选择不同浓度的松果菊苷处理卵巢癌SKOV3细胞,24 h后CCK8检测细胞存活率;选择不同浓度松果菊苷浓度(0,20,40,80μmol·L-1)进行后续实验,EdU染色检测细胞增殖;Transwell检测细胞侵袭;免疫印迹法检测VEGF、E-cadherin、Vimentin的表达及p38 MAPK的磷酸化;显微观察细胞肿瘤球形态;流式细胞仪检测CD133和CD44的含量。结果 SKOV3细胞的存活率与松果菊苷呈浓度依赖性降低,松果菊苷浓度≥40μmol·L-1时,SKOV3细胞的存活率较松果菊苷0μmol·L-1时均明显降低(P<0.05)。与松果菊苷0μmol·L-1组比较,松果菊苷40μmol·L-1组和80μmol·L-1组EdU阳性细胞数和侵袭细胞数均减少(P<0.05);VEGF和Vimentin蛋白表达水平及p38 MAPK磷酸化水平均降低(P<0.05);E-cadherin蛋白表达水平升高(P<0.05);肿瘤干细胞成球直径减小(P<0.05),成球数减少(P<0.05);CD133和CD44表达均降低(P<0.05)。结论 松果菊苷可以抑制卵巢癌SKOV3细胞的增殖、侵袭、干细胞样特性及p38 MAPK的磷酸化。 |
| 关键词: 松果菊苷 p38MAPK 卵巢癌 增殖 侵袭 干细胞样特性 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.10.005 |
| 分类号:R285.5 |
| 基金项目:2021年度河南省卫生和计划生育委员会教改项目(Wjlx2021019);黄河科技学院2019年课程改革与实践项目(kg2019a105);河南省二O一七年科技发展计划项目(172102310528) |
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| Echinacoside Inhibits the Proliferation, Invasion and Stem Cell-like Properties of Ovarian Cancer SKOV3 Cells |
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DONG Ya-na1, GUO Bao-zhong1, HOU Jing-jing1, YUAN Zhen-ying2, TANG Ya-hui1, YANG Juan1
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1.Huanghe University of Science and Technology, Zhengzhou 450000, China;2.Zhengzhou First People's Hospital, Zhengzhou 450000, China
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| Abstract: |
| OBJECTIVE To explore the effects of echinacoside on the ovarian cancer SKOV3 cells.METHODS Different doses of echinacoside were used to treat ovarian cancer SKOV3 cells,and cell survival rate was detected by CCK8 after 24 h.Different echinacoside concentration(0,20,40,80μmol·L-1) were selected for subsequent experiments,and EdU staining was used to detect cell proliferation;Transwell was employed to detect cell invasion;Western blotting was commended to detect the expression of VEGF,E-cadherin,Vimentin and p38 MAPK phosphorylation level.Microscopically observed the tumor spheroids.Flow cytometry was performed to detect the content of CD133 and CD44.RESULTS The survival rate of SKOV3 cells decreased in a concentration-dependent manner,when echinacoside concentration ≥ 40μmol·L-1,the survival rate of SKOV3 cells was significantly lower than that of echinacoside 0μmol·L-1(P<0.05).Compared with the echinacoside0μmol·L-1 group,the number of EdU-positive cells and invasion cells in the echinacoside 40μmol·L-1 group and echinacoside80μmol·L-1 group were significantly reduced(P<0.05);the expression of VEGF,Vimentin protein and p38 MAPK phosphorylation level were significantly reduced(P<0.05);the expression level of E-cadherin protein was significantly increased(P<0.05);tumor stem cell bulb diameter decreased significantly(P<0.05),and the number of bulb formation decreased significantly(P<0.05);the expression of CD133 and CD44 were significantly reduced(P<0.05).CONCLUSION Echinacoside can inhibit the proliferation,invasion,stem cell-like properties and the p38 MAPK phosphorylation of ovarian cancer SKOV3 cells. |
| Key words: echinacoside p38 MAPK ovarian cancer proliferation invasion stem cell-like properties |
