| 引用本文: | 程巧鸳,施诗,周明昊,吴鸳鸯.基于UPLC-Q-TOF-MSn的化妆品中40种性激素非法添加分析方法及筛查数据库的建立[J].中国现代应用药学,2022,39(5):614-621. |
| CHENG Qiaoyuan,SHI Shi,ZHOU Minghao,WU Yuanyang.Establishment of an Analysis Method and Screening Database for Illegal Addition of 40 Sex Hormones in Cosmetics Based on UPLC-Q-TOF-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(5):614-621. |
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| 摘要: |
| 目的 建立基于UPLC-Q-TOF-MSn的化妆品中40种性激素非法添加分析方法及筛查数据库。方法 色谱柱为Agilent SB-C18(2.1 mm×50 mm，1.8 μm)，正离子模式以0.1%甲酸水溶液-乙腈为流动相，负离子模式以水-乙腈为流动相，梯度洗脱，流速0.2 mL·min-1，柱温35 ℃；采用电喷雾电离源(ESI)，采集模式为Scan MS及Targeted MS/MS，将40种性激素的精确质量数、保留时间、二级质谱图导入PCDL软件建立筛查数据库。结果 采用所建立的数据库方法快速筛查100批化妆品，1批育发类产品检出少量黄体酮。结论 本研究所建立的40种性激素分析方法和筛查数据库具有简便、快速、灵敏和准确的特点，适合化妆品中非法添加性激素的高通量筛查和准确定性。 |
| 关键词: 化妆品 非法添加 性激素 超高效液相色谱-飞行时间质谱 筛查数据库 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.05.007 |
| 分类号:R917.101 |
| 基金项目:浙江省药品监管系统科技计划项目（2021036） |
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| Establishment of an Analysis Method and Screening Database for Illegal Addition of 40 Sex Hormones in Cosmetics Based on UPLC-Q-TOF-MS |
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CHENG Qiaoyuan1, SHI Shi2, ZHOU Minghao1, WU Yuanyang1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Zhejiang Chinese Medical University, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To establish an analysis method and screening database based on UPLC-Q-TOF-MSn for illegal addition of 40 sex hormones in cosmetics. METHODS The gradient elution was carried out on Agilent SB-C18 column (2.1 mm×50 mm, 1.8 μm), 0.1% formic acid water and acetonitrile were used as mobile phase in positive ion mode, water and acetonitrile were used as mobile phase in negative ion mode, the flow rate was 0.2 mL·min-1, the column temperature was 35℃. MS detection was carried out by electrospray ionization(ESI)mass spectrometer in Scan MS and Targeted MS/MS mode. The accurate mass, retention time and MS/MS mass spectrum of 40 sex hormones were imported into PCDL software to establish a rapid screening database. RESULTS The established method and database was used to screen 100 batches of cosmetics, and one batch of hair-growing product was detected with a small amount of progesterone. CONCLUSION The established analysis method and screening database including 40 sex hormones is fast, sensitive and accurate, which is suitable for high-throughput screening and accurate qualitative analysis of sex hormones adulterants in cosmetics. |
| Key words: cosmetics illegal addition sex hormones UPLC-Q-TOF-MSn screening database |
