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引用本文:杨月娥,陶汝晋,刘玉雨,李国平,赵若丹,黄志莉,张海珠,段宝忠.3种重楼属药用植物的生药学研究[J].中国现代应用药学,2021,38(22):2775-2780.
YANG Yue'e,TAO Rujin,LIU Yuyu,LI Guoping,ZHAO Ruodan,HUANG Zhili,ZHANG Haizhu,DUAN Baozhong.Pharmacognostical Study on Three Medicinal Plants of Genus Paris[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(22):2775-2780.
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3种重楼属药用植物的生药学研究
杨月娥1, 陶汝晋1, 刘玉雨1, 李国平1, 赵若丹1, 黄志莉1, 张海珠1,2, 段宝忠1,2
1.大理大学药学院, 云南 大理 671000;2.云南省高校滇西道地药材资源开发重点实验室, 云南 大理 671000
摘要:
目的 对腾冲重楼、大理重楼、漕涧重楼3种药用植物进行系统的生药学研究。方法 采用原植物观察、石蜡切片、水合氯醛透化法和HPLC,对腾冲重楼等3种重楼属药用植物进行系统研究。结果 3种重楼属药用植物的原植物形态差异明显,但性状、根茎横切面和粉末显微特征较为相似;腾冲重楼中含有重楼皂苷VII、重楼皂苷VI、重楼皂苷II、重楼皂苷III、纤细薯蓣皂苷、重楼皂苷I等6种成分,漕涧重楼和大理重楼仅检测到重楼皂苷VII和重楼皂苷VI。结论 显微鉴别无法有效区分3种植物的药用部位,但3种药用植物化学成分差异明显,HPLC可作为3种重楼鉴别的有效手段。
关键词:  腾冲重楼  漕涧重楼  大理重楼  生药学  高效液相色谱法  重楼皂苷
DOI:10.13748/j.cnki.issn1007-7693.2021.22.002
分类号:R284.1
基金项目:国家自然科学基金项目(31860080);云南省重大科技专项(202002AA100007);云南省院士专家工作站(202105AF150053)
Pharmacognostical Study on Three Medicinal Plants of Genus Paris
YANG Yue'e1, TAO Rujin1, LIU Yuyu1, LI Guoping1, ZHAO Ruodan1, HUANG Zhili1, ZHANG Haizhu1,2, DUAN Baozhong1,2
1.College of Pharmaceutical Science, Dali University, Dali 671000, China;2.Yunnan Key University Laboratory of Genuine Medicinal Materials Resources Development in West Yunnan, Dali 671000, China
Abstract:
OBJECTIVE To carry out systematic pharmacognostical research on 3 kinds of medicinal materials:Paris tengchongensis, Paris daliensis and Paris caojianensis. METHODS The original plant observation, paraffin section, chloral hydrate permeation method, and HPLC were used to systematically study 3 medicinal plants of Genus Paris. RESULTS The morphology of the original plants was significantly different, but the characteristics of the medicinal materials, the cross-section of the rhizome and the microscopic characteristics of the powder were similar. There were 6 kinds of ingredients, such as polyphyllin VII, polyphyllin VI, polyphyllin II, polyphyllin III, gracillin and polyphyllin I were detected in Paris tengchongensis. Only polyphyllin VII and polyphyllin VI were detected in Paris caojianensis and Paris daliensis. CONCLUSION Microscopic identification cannot distinguish the three kinds of Paris effectively, but the HPLC of the 3 species are significantly different, which can be used as an effective method for the identification of three kinds of Paris.
Key words:  Paris tengchongensis  Paris caojianensis  Paris daliensis  pharmacognosy  HPLC  polyphyllin
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