| 引用本文: | 刘敬,廉博雯,黄晓婷,赖石凤,赵斌,汪保国,梅文杰.毛兰素对斑马鱼神经行为的改变研究[J].中国现代应用药学,2021,38(14):1671-1677. |
| LIU Jing,LIAN Bowen,HUANG Xiaoting,LAI Shifeng,ZHAO Bin,WANG Baoguo,MEI Wenjie.Effect of Erianin on Neuro-behavioral Alterations of Zebrafish[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(14):1671-1677. |
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| 毛兰素对斑马鱼神经行为的改变研究 |
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刘敬1, 廉博雯2, 黄晓婷2, 赖石凤2, 赵斌3, 汪保国2,4, 梅文杰2,4
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1.中山火炬职业技术学院健康产业学院/国家中药现代化工程技术研究中心中山健康产品分中心, 广东 中山 528436;2.广东药科大学公共健康学院/广东药科大学药学院, 广州 510006;3.广东江门中医药职业学院南药学院, 广东 江门 509099;4.广东分子探针与生物医学成像工程技术研究中心, 广州 510006
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| 摘要: |
| 目的 探讨石斛活性成分毛兰素对斑马鱼神经行为的改变作用。方法 通过胚胎行为分析、幼体运动活性测定、胚胎凋亡检测等研究毛兰素对斑马鱼胚胎和幼鱼神经发育的调节作用；通过胚胎发育毒性评价、组织生长试验研究毛兰素对胚胎发育的影响。结果 毛兰素能明显促进斑马鱼的自发活动，缓解神经抑制状态，提高斑马鱼的适应能力；同时毛兰素还抑制了胚胎的孵化，具体表现为卵黄囊增大，卵黄吸收降低，体长缩短。结论 毛兰素是一种具有良好前景的神经保护的药物，但同时还应考虑其毒性作用。 |
| 关键词: 毛兰素 毒性 神经行为 细胞凋亡 斑马鱼 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.14.002 |
| 分类号:R285.5 |
| 基金项目:中山市科技发展专项项目（2014A2FC244）；广东省普通高校特色创新项目（2019GKTSCX101） |
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| Effect of Erianin on Neuro-behavioral Alterations of Zebrafish |
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LIU Jing1, LIAN Bowen2, HUANG Xiaoting2, LAI Shifeng2, ZHAO Bin3, WANG Baoguo2,4, MEI Wenjie2,4
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1.Department of of Health Industry, Zhongshan Torch Polytechnic/Zhongshan Health Products Branch, National Engineering Research Center for Modernization of Traditional Chinese Medicine, Zhongshan 528436, China;2.School of Public Health, Guangdong Pharmaceutical University/School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;3.Department of Southern Pharmacy, Guangdong Jiangmen Chinese Medical College, Jiangmen 509099, China;4.Guangdong Province Engineering Technology Centre for Molecular Probe and Biomedicine Imaging, Guangzhou 510006, China
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| Abstract: |
| OBJECTIVE To explore the effect of erianin, an active component of Dendrobii Caulis, on the neuro-behavior of zebrafish. METHODS Erianin's regulation of zebrafish embryo and larval neurodevelopment was studied through embryo behavior analysis, larval locomotor activity determination, and embryo apoptosis detection. Meantime, embryo development toxicity evaluation and tissue growth test were used to study the effect of erianin on embryo development. RESULTS Erianin significantly accelerated the spontaneous movement, relieved the neuro inhibition condition and increased the acclimation capacity of zebrafish. In addition, erianin inhibited the hatchability, impaired yolk absorption as yolk sac area increasing, and reduced body length. CONCLUSION Erianin can be considered as a promising agent for treating neurological diseases in the future, meanwhile the toxic effects should be taken into account. |
| Key words: erianin toxicity neuro-behaviors apoptosis zebrafish |
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